Bovine mitochondrial initiation and elongation factors.
Journal Article (Journal Article)
The procedures summarized above provide nearly homogeneous preparations of IF-2mt, EF-Tu. Tsmt, and EF-Gmt. The scheme developed for IF-2mr leads to a 24,000-fold purification of this factor with a 26% recovery of activity. Analysis by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography indicates that this factor functions as a monomer with a molecular weight of about 85,000. The scheme developed EF-Tu.Tsmt provides a 10,000-fold purification with an overall yield of about 10%. The EF-Tumt component in this complex has a molecular weight of about 46,000, whereas EF-Tsmt has a molecular weight of about 32,000 on SDS-polyacrylamide gel electrophoresis. The EF-Tu. Tsmt complex is tightly associated and appears to have a native molecular weight of about 70,000. The five-step purification procedure outlined above for EF-Gmt results in a 14,000-fold purification of EF-Gmt with a 2-5% recovery of activity. Analysis by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography indicates that EF-Gmt functions as a monomeric protein with an apparent molecular weight of about 80,000.
Full Text
Duke Authors
Cited Authors
- Schwartzbach, CJ; Farwell, M; Liao, HX; Spremulli, LL
Published Date
- 1996
Published In
Volume / Issue
- 264 /
Start / End Page
- 248 - 261
PubMed ID
- 8965699
International Standard Serial Number (ISSN)
- 0076-6879
Digital Object Identifier (DOI)
- 10.1016/s0076-6879(96)64025-7
Language
- eng
Conference Location
- United States