Bovine mitochondrial initiation and elongation factors.

Published

Journal Article

The procedures summarized above provide nearly homogeneous preparations of IF-2mt, EF-Tu. Tsmt, and EF-Gmt. The scheme developed for IF-2mr leads to a 24,000-fold purification of this factor with a 26% recovery of activity. Analysis by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography indicates that this factor functions as a monomer with a molecular weight of about 85,000. The scheme developed EF-Tu.Tsmt provides a 10,000-fold purification with an overall yield of about 10%. The EF-Tumt component in this complex has a molecular weight of about 46,000, whereas EF-Tsmt has a molecular weight of about 32,000 on SDS-polyacrylamide gel electrophoresis. The EF-Tu. Tsmt complex is tightly associated and appears to have a native molecular weight of about 70,000. The five-step purification procedure outlined above for EF-Gmt results in a 14,000-fold purification of EF-Gmt with a 2-5% recovery of activity. Analysis by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography indicates that EF-Gmt functions as a monomeric protein with an apparent molecular weight of about 80,000.

Full Text

Duke Authors

Cited Authors

  • Schwartzbach, CJ; Farwell, M; Liao, HX; Spremulli, LL

Published Date

  • January 1, 1996

Published In

Volume / Issue

  • 264 /

Start / End Page

  • 248 - 261

PubMed ID

  • 8965699

Pubmed Central ID

  • 8965699

Electronic International Standard Serial Number (EISSN)

  • 1557-7988

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/s0076-6879(96)64025-7

Language

  • eng