Identification of human erythrocyte blood group antigens on the C3b/C4b receptor.
Journal Article (Journal Article)
The Knops/McCoy (Kn/McC) human erythrocyte blood group system belongs to the category of blood group Ag that generate so-called "high titer low avidity" antibodies in immunized transfusion recipients. Screening of red cells lacking certain high titer low avidity Ag demonstrated markedly diminished CR1 expression on McC(d-) and Kn/McC "null" (Kn(a-)McC(a-b-c-d-e-f-] erythrocytes. Additional testing by other methods confirmed these data, and biochemical assays demonstrated no detectable immunoreactive CR1 protein in membranes from Kn/McC null red cells. Human antisera to various Kn/McC Ag were then used to demonstrate that many of these antisera could be used to isolate a protein of identical m.w. to that isolated from the same cells using murine mAb CR1 antisera. Finally, protein isolated by using murine mAb anti-CR1 reacted specifically with anti-Kn/McC antibodies, demonstrating the identity of the Kn/McC and CR1 proteins. Thus, CR1 protein bears the human erythrocyte Kn/McC blood group Ag.
Full Text
Duke Authors
Cited Authors
- Rao, N; Ferguson, DJ; Lee, SF; Telen, MJ
Published Date
- May 15, 1991
Published In
Volume / Issue
- 146 / 10
Start / End Page
- 3502 - 3507
PubMed ID
- 1827486
International Standard Serial Number (ISSN)
- 0022-1767
Language
- eng
Conference Location
- United States