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Monoclonal antibody recognizing a unique Rh-related specificity.

Publication ,  Journal Article
Poss, MT; Swanson, JL; Telen, MJ; Lasky, LC; Vallera, DA
Published in: Vox Sang
1993

A mouse IgG1 monoclonal antibody (MAb) UMRh, was prepared by immunizing Balb/c mice with the Jurkat T cell acute lymphoblastic leukemia (T-ALL) cell line. The MAb UMRh is directed against a widely distributed Rh-related cell surface antigen, present on red blood cells (RBCs) expressing the more common Rh phenotypes. The antigen has reduced expression on RBCs of -D-, DCW-/DCW-, Rhmod and Rhnull phenotypes. UMR immunoblotted a unique pattern on RBC membrane preparations of two bands at 40 and 43 kD and a diffuse pattern extending upward to about 55 kD. The UMRh antigen is also present on peripheral blood mononuclear cells, granulocytes, platelets, leukemic cells of T cell, B cell and myeloid origins, hematopoietic stem cells, and two tumor lines (lung and colon carcinoma). The number of UMRh sites per RBC (CDe/ce) was determined to be 5,519 copies/cell, whereas the sites on a -D- phenotype RBC were 1,096 copies/cell. A T-ALL line (CEM) expressed 333,364 copies/cell and a myeloid line (KG-1) expressed 90,913 copies/cell. Several Rh-related murine MAbs have been described, but our data indicates that UMRh recognizes a previously uncharacterized Rh-related specificity.

Duke Scholars

Published In

Vox Sang

DOI

ISSN

0042-9007

Publication Date

1993

Volume

64

Issue

4

Start / End Page

231 / 239

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Serology
  • Rh-Hr Blood-Group System
  • Mice, Inbred BALB C
  • Mice
  • Leukemia-Lymphoma, Adult T-Cell
  • Immunoglobulin G
  • Humans
  • Flow Cytometry
  • Epitopes
 

Citation

APA
Chicago
ICMJE
MLA
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Poss, M. T., Swanson, J. L., Telen, M. J., Lasky, L. C., & Vallera, D. A. (1993). Monoclonal antibody recognizing a unique Rh-related specificity. Vox Sang, 64(4), 231–239. https://doi.org/10.1111/j.1423-0410.1993.tb03061.x
Poss, M. T., J. L. Swanson, M. J. Telen, L. C. Lasky, and D. A. Vallera. “Monoclonal antibody recognizing a unique Rh-related specificity.Vox Sang 64, no. 4 (1993): 231–39. https://doi.org/10.1111/j.1423-0410.1993.tb03061.x.
Poss MT, Swanson JL, Telen MJ, Lasky LC, Vallera DA. Monoclonal antibody recognizing a unique Rh-related specificity. Vox Sang. 1993;64(4):231–9.
Poss, M. T., et al. “Monoclonal antibody recognizing a unique Rh-related specificity.Vox Sang, vol. 64, no. 4, 1993, pp. 231–39. Pubmed, doi:10.1111/j.1423-0410.1993.tb03061.x.
Poss MT, Swanson JL, Telen MJ, Lasky LC, Vallera DA. Monoclonal antibody recognizing a unique Rh-related specificity. Vox Sang. 1993;64(4):231–239.
Journal cover image

Published In

Vox Sang

DOI

ISSN

0042-9007

Publication Date

1993

Volume

64

Issue

4

Start / End Page

231 / 239

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Serology
  • Rh-Hr Blood-Group System
  • Mice, Inbred BALB C
  • Mice
  • Leukemia-Lymphoma, Adult T-Cell
  • Immunoglobulin G
  • Humans
  • Flow Cytometry
  • Epitopes