Isolation of the JMH antigen on a novel phosphatidylinositol-linked human membrane protein.

Published

Journal Article

JMH is a high-frequency human erythrocyte blood group antigen. Previous work has shown that JMH is absent from complement-sensitive erythrocytes of patients with paroxysmal nocturnal hemoglobinuria (PNH); such cells have a broad defect in expression of phosphatidylinositol (PI)-linked proteins. Using both human JMH antisera and a JMH-like murine monoclonal antibody, we have identified a 76-Kd membrane protein present in JMH-positive but not JMH-negative erythrocytes. A similar 76-Kd JMH protein was also identified on a human lymphoid T-cell line, HSB-2. Using PI-specific phospholipase C, a small amount of JMH antigen could be cleaved from intact erythrocytes and immunoprecipitated from the supernate of treated erythrocytes, thus confirming that the protein bearing the JMH antigen is anchored by a PI-linkage to the erythrocyte membrane. This protein was further shown not to be identical to decay accelerating factor (70 Kd), a previously identified PI-anchored protein of somewhat similar molecular weight.

Full Text

Duke Authors

Cited Authors

  • Bobolis, KA; Moulds, JJ; Telen, MJ

Published Date

  • March 15, 1992

Published In

Volume / Issue

  • 79 / 6

Start / End Page

  • 1574 - 1581

PubMed ID

  • 1372190

Pubmed Central ID

  • 1372190

International Standard Serial Number (ISSN)

  • 0006-4971

Language

  • eng

Conference Location

  • United States