Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment.


Journal Article

When the picrosirius red technique was applied to cardiac muscle sections, intense yellow myocyte staining sometimes obscured thin collagenous septa. The picrosirius red technique was modified to include treatment of the sections in 0.2% (w/v) aqueous phosphomolybdic acid prior to staining. With 1-5 min treatment, cytoplasmic staining was eradicated; diminution of collagen staining occurred only with long treatments at much higher concentrations of phosphomolybdic acid. Using this phosphomolybdic acid-picrosirius red technique, collagenous septa as thin as 0.2-0.5 micron and fine collagen fibers making up the septa were clearly discernible. The technique also worked well on sections stained by other techniques and then destained. The phosphomolybdic acid-picrosirius red technique should be useful in experiments designed to investigate the effects of collagen distribution on the electrical and mechanical behavior of cardiac muscle.

Full Text

Cited Authors

  • Dolber, PC; Spach, MS

Published Date

  • January 1987

Published In

Volume / Issue

  • 62 / 1

Start / End Page

  • 23 - 26

PubMed ID

  • 2438817

Pubmed Central ID

  • 2438817

International Standard Serial Number (ISSN)

  • 0038-9153

Digital Object Identifier (DOI)

  • 10.3109/10520298709107961


  • eng