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Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice.

Publication ,  Journal Article
Linko, P; Yeowell, HN; Gasiewicz, TA; Goldstein, JA
Published in: J Biochem Toxicol
June 1986

Hexachlorobenzene (HCB) differs markedly from other chlorinated benzenes (CBs) as an inducer of cytochrome P-450 (P-450) isozymes as determined by radioimmunoassay and immunoblotting. At greater than 99% pure, HCB induced both the phenobarbital-inducible forms, cytochromes P-450b + e (70 chi), and the 3-methylcholanthrene-inducible forms, cytochromes P-450c (58 chi) and P-450d (8 chi), in rat liver microsomes. The concentration of P-450d was considerably greater than that of P-450c in HCB-induced rat liver. In contrast to HCB, all lower chlorinated benzenes tested were PB-type inducers. Hexachlorobenzene increased the amounts of translatable messenger RNAs (mRNAs) for P-450b, P-450c, and P-450d in rat liver polysomes, suggesting that it increases the synthesis of these proteins. Evidence that HCB interacted with the putative Ah receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was equivocal. Western blots of liver microsomes from Ah-responsive C57BL/6J (B6) and nonresponsive DBA/2J (D2) mice demonstrated that HCB produced a large increase in P3-450 and a very small increase in P1-450 in the responsive strain. The increase in P1-450 was not observed after HCB administration to nonresponsive mice, but a small increase in P3-450 was noted. These findings suggested that HCB may act through the Ah receptor. However, HCB was at best a very weak competitor for specific binding of [3H]-TCDD to the putative receptor in rat or mouse hepatic cytosol in vitro, producing decreases in binding of [3H]-TCDD only at very high concentrations (10(-6) to 10(-5) M).

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Published In

J Biochem Toxicol

DOI

ISSN

0887-2082

Publication Date

June 1986

Volume

1

Issue

2

Start / End Page

95 / 107

Location

United States

Related Subject Headings

  • Receptors, Drug
  • Receptors, Aryl Hydrocarbon
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Protein Biosynthesis
  • Polyribosomes
  • Polychlorinated Dibenzodioxins
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
 

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Linko, P., Yeowell, H. N., Gasiewicz, T. A., & Goldstein, J. A. (1986). Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice. J Biochem Toxicol, 1(2), 95–107. https://doi.org/10.1002/jbt.2570010209
Linko, P., H. N. Yeowell, T. A. Gasiewicz, and J. A. Goldstein. “Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice.J Biochem Toxicol 1, no. 2 (June 1986): 95–107. https://doi.org/10.1002/jbt.2570010209.
Linko P, Yeowell HN, Gasiewicz TA, Goldstein JA. Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice. J Biochem Toxicol. 1986 Jun;1(2):95–107.
Linko, P., et al. “Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice.J Biochem Toxicol, vol. 1, no. 2, June 1986, pp. 95–107. Pubmed, doi:10.1002/jbt.2570010209.
Linko P, Yeowell HN, Gasiewicz TA, Goldstein JA. Induction of cytochrome P-450 isozymes by hexachlorobenzene in rats and aromatic hydrocarbon (Ah)-responsive mice. J Biochem Toxicol. 1986 Jun;1(2):95–107.

Published In

J Biochem Toxicol

DOI

ISSN

0887-2082

Publication Date

June 1986

Volume

1

Issue

2

Start / End Page

95 / 107

Location

United States

Related Subject Headings

  • Receptors, Drug
  • Receptors, Aryl Hydrocarbon
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Protein Biosynthesis
  • Polyribosomes
  • Polychlorinated Dibenzodioxins
  • Mice, Inbred DBA
  • Mice, Inbred C57BL