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Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues.

Publication ,  Journal Article
Canada, AT; Roberson, KM; Vessella, RL; Trump, DL; Robertson, CN; Fine, RL
Published in: Biochem Pharmacol
January 12, 1996

Metastatic prostate adenocarcinoma is unresponsive to alkylator chemotherapy with virtually no prolonged remissions. Glutathione (GSH) and glutathione S-transferase (GST) have been reported to play a role in tumor resistance to alkylator therapy; however, there are no baseline studies that have investigated and compared GSH and GST in human prostate cell lines and tissues. Thus, we determined the GSH content and GST activity in benign prostate, in primary and metastatic prostate adenocarcinoma tissues, in immortal adenocarcinoma cell lines, and in primary cell cultures derived from both benign prostate and primary prostatic carcinoma tissue. The GSH content was higher in the immortal cell lines than in the fresh tissues and primary cultures. Conversely, the GST activity was significantly higher in the tissues and primary cultures than in the cell lines. The GSH content and GST activity of the primary cultured prostatic cells were similar to those of the prostate tissues. The differences between the immortal prostate cancer cell lines and prostate tissue are of sufficient magnitude to suggest that in vitro results with cell lines may not extrapolate to prostate cancer in vivo. The GSH content and GST activity in a prostate specific antigen-secreting human prostate tumor xenograft, LuCaP23, maintained in nude mice were similar to those of human prostate tissue and primary cultures. Both the xenograft and primary cultures from patients with prostate cancer may be more appropriate models than established cell lines for investigating techniques to increase the effectiveness of alkylators in prostate cancer.

Duke Scholars

Published In

Biochem Pharmacol

DOI

ISSN

0006-2952

Publication Date

January 12, 1996

Volume

51

Issue

1

Start / End Page

87 / 90

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Prostatic Neoplasms
  • Prostate
  • Pharmacology & Pharmacy
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Humans
 

Citation

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Canada, A. T., Roberson, K. M., Vessella, R. L., Trump, D. L., Robertson, C. N., & Fine, R. L. (1996). Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues. Biochem Pharmacol, 51(1), 87–90. https://doi.org/10.1016/0006-2952(95)02157-4
Canada, A. T., K. M. Roberson, R. L. Vessella, D. L. Trump, C. N. Robertson, and R. L. Fine. “Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues.Biochem Pharmacol 51, no. 1 (January 12, 1996): 87–90. https://doi.org/10.1016/0006-2952(95)02157-4.
Canada AT, Roberson KM, Vessella RL, Trump DL, Robertson CN, Fine RL. Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues. Biochem Pharmacol. 1996 Jan 12;51(1):87–90.
Canada, A. T., et al. “Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues.Biochem Pharmacol, vol. 51, no. 1, Jan. 1996, pp. 87–90. Pubmed, doi:10.1016/0006-2952(95)02157-4.
Canada AT, Roberson KM, Vessella RL, Trump DL, Robertson CN, Fine RL. Glutathione and glutathione S-transferase in benign and malignant prostate cell lines and prostate tissues. Biochem Pharmacol. 1996 Jan 12;51(1):87–90.
Journal cover image

Published In

Biochem Pharmacol

DOI

ISSN

0006-2952

Publication Date

January 12, 1996

Volume

51

Issue

1

Start / End Page

87 / 90

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Prostatic Neoplasms
  • Prostate
  • Pharmacology & Pharmacy
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Humans