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Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping.

Publication ,  Journal Article
Padilla-Nash, HM; Nash, WG; Padilla, GM; Roberson, KM; Robertson, CN; Macville, M; Schröck, E; Ried, T
Published in: Genes Chromosomes Cancer
May 1999

The bladder cancer cell line BK-10 was established from a grade III-IV transitional cell carcinoma (TCC). BK-10 is near-tetraploid (+/-4n) and consists of two subclones with 20-25 structural aberrations. Here we report the cytogenetic analysis of BK-10 by G-banding, spectral karyotyping (SKY), and FISH. SKY refers to the hybridization of 24 differentially labeled chromosome painting probes and the simultaneous visualization of all human chromosomes using spectral imaging. SKY enabled us to confirm 12 markers in BK-10 previously described by G-banding, redefine 11 aberrations, and detect 4 hidden chromosomal rearrangements, 2 of which had been identified as normal or deleted copies of chromosome 20 and 1 as a normal chromosome 3. Twenty out of 21 translocations identified were unbalanced. FISH analysis of BK-10 using chromosome arm-specific paints, centromere probes, and oncogene/tumor suppressor gene-specific probes revealed a deletion of CDKN2A (p16) in all copies of chromosome 9, a low-level amplification of MYC (five copies), and loss of one copy of TP53; detected the presence of the Y chromosome in a hidden translocation; and detected four copies of ERBB-2. A probe set for BCR and ABL verified breakpoints for all translocations involving chromosomes 9 and 22. A new karyotype presentation, "SKY-gram," is introduced by combining data from G-banding, SKY, and FISH analysis. This study demonstrates the approach of combining molecular cytogenetic techniques to characterize fully the multiple complex chromosomal rearrangements found in the bladder cancer cell line BK-10, and to refine the chromosomal breakpoints for all translocations.

Duke Scholars

Published In

Genes Chromosomes Cancer

DOI

ISSN

1045-2257

Publication Date

May 1999

Volume

25

Issue

1

Start / End Page

53 / 59

Location

United States

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Male
  • Karyotyping
  • In Situ Hybridization, Fluorescence
  • Humans
  • Chromosome Painting
  • Chromosome Banding
  • Carcinoma
 

Citation

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MLA
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Padilla-Nash, H. M., Nash, W. G., Padilla, G. M., Roberson, K. M., Robertson, C. N., Macville, M., … Ried, T. (1999). Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping. Genes Chromosomes Cancer, 25(1), 53–59. https://doi.org/10.1002/(sici)1098-2264(199905)25:1<53::aid-gcc8>3.0.co;2-t
Padilla-Nash, H. M., W. G. Nash, G. M. Padilla, K. M. Roberson, C. N. Robertson, M. Macville, E. Schröck, and T. Ried. “Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping.Genes Chromosomes Cancer 25, no. 1 (May 1999): 53–59. https://doi.org/10.1002/(sici)1098-2264(199905)25:1<53::aid-gcc8>3.0.co;2-t.
Padilla-Nash HM, Nash WG, Padilla GM, Roberson KM, Robertson CN, Macville M, et al. Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping. Genes Chromosomes Cancer. 1999 May;25(1):53–9.
Padilla-Nash, H. M., et al. “Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping.Genes Chromosomes Cancer, vol. 25, no. 1, May 1999, pp. 53–59. Pubmed, doi:10.1002/(sici)1098-2264(199905)25:1<53::aid-gcc8>3.0.co;2-t.
Padilla-Nash HM, Nash WG, Padilla GM, Roberson KM, Robertson CN, Macville M, Schröck E, Ried T. Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping. Genes Chromosomes Cancer. 1999 May;25(1):53–59.
Journal cover image

Published In

Genes Chromosomes Cancer

DOI

ISSN

1045-2257

Publication Date

May 1999

Volume

25

Issue

1

Start / End Page

53 / 59

Location

United States

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Male
  • Karyotyping
  • In Situ Hybridization, Fluorescence
  • Humans
  • Chromosome Painting
  • Chromosome Banding
  • Carcinoma