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Isolation and characterization of a novel human bladder cancer cell line: BK10.

Publication ,  Journal Article
Roberson, KM; Yancey, DR; Padilla-Nash, H; Edwards, DW; Nash, W; Jacobs, S; Padilla, GM; Larchian, WA; Robertson, CN
Published in: In Vitro Cell Dev Biol Anim
1998

Molecular studies of bladder carcinomas have aided in determining causative genetic events and the prognosis of cancers endowed with certain abnormalities. In vitro bladder cancer characterization of key cytogenetic alterations is useful for study of molecular changes that may promote oncogenic events. In our laboratory, a novel human bladder cancer cell line, BK10, has been established in vitro and passaged for more than 20 mo. This new bladder cancer cell line (BK10) was derived from bladder tissue containing grade III-IV/IV transitional cell carcinoma. Bladder cancer tissue was obtained at the time of radical cystoprostatectomy extirpation. Cell cultures derived from this surgical sample exhibited an epithelial morphology and expressed epithelial cytokeratins. Immunostains of BK10 were negative for prostate specific antigen (PSA), fibronectin, smooth muscle actin alpha, and desmin. Karyotypic analysis revealed an aneuploid chromosomal content <4n> with many numerical and structural abnormalities previously linked to bladder oncogenesis. Translocations occurred in chromosomes 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 13, 14, 15, 16, 17, 19, 20, 21, 22, X and Y. G-banding analysis revealed rearrangements involving chromosomes 9q and 17p, and the location of the ab11 oncogene and the p53 gene, respectively. The availability of this bladder cancer cell line will provide a useful tool for the further study of bladder carcinoma oncogenesis and gene therapy.

Duke Scholars

Published In

In Vitro Cell Dev Biol Anim

DOI

ISSN

1071-2690

Publication Date

1998

Volume

34

Issue

7

Start / End Page

537 / 544

Location

Germany

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Tumor Cells, Cultured
  • Mice, Nude
  • Mice
  • Male
  • Karyotyping
  • Immunohistochemistry
  • Humans
  • Cell Division
  • Carcinoma, Transitional Cell
 

Citation

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Roberson, K. M., Yancey, D. R., Padilla-Nash, H., Edwards, D. W., Nash, W., Jacobs, S., … Robertson, C. N. (1998). Isolation and characterization of a novel human bladder cancer cell line: BK10. In Vitro Cell Dev Biol Anim, 34(7), 537–544. https://doi.org/10.1007/s11626-998-0113-y
Roberson, K. M., D. R. Yancey, H. Padilla-Nash, D. W. Edwards, W. Nash, S. Jacobs, G. M. Padilla, W. A. Larchian, and C. N. Robertson. “Isolation and characterization of a novel human bladder cancer cell line: BK10.In Vitro Cell Dev Biol Anim 34, no. 7 (1998): 537–44. https://doi.org/10.1007/s11626-998-0113-y.
Roberson KM, Yancey DR, Padilla-Nash H, Edwards DW, Nash W, Jacobs S, et al. Isolation and characterization of a novel human bladder cancer cell line: BK10. In Vitro Cell Dev Biol Anim. 1998;34(7):537–44.
Roberson, K. M., et al. “Isolation and characterization of a novel human bladder cancer cell line: BK10.In Vitro Cell Dev Biol Anim, vol. 34, no. 7, 1998, pp. 537–44. Pubmed, doi:10.1007/s11626-998-0113-y.
Roberson KM, Yancey DR, Padilla-Nash H, Edwards DW, Nash W, Jacobs S, Padilla GM, Larchian WA, Robertson CN. Isolation and characterization of a novel human bladder cancer cell line: BK10. In Vitro Cell Dev Biol Anim. 1998;34(7):537–544.
Journal cover image

Published In

In Vitro Cell Dev Biol Anim

DOI

ISSN

1071-2690

Publication Date

1998

Volume

34

Issue

7

Start / End Page

537 / 544

Location

Germany

Related Subject Headings

  • Urinary Bladder Neoplasms
  • Tumor Cells, Cultured
  • Mice, Nude
  • Mice
  • Male
  • Karyotyping
  • Immunohistochemistry
  • Humans
  • Cell Division
  • Carcinoma, Transitional Cell