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Chemoattractant receptor-induced phosphorylation of L-selectin.

Publication ,  Journal Article
Haribabu, B; Steeber, DA; Ali, H; Richardson, RM; Snyderman, R; Tedder, TF
Published in: J Biol Chem
May 23, 1997

The selectin adhesion molecules and chemoattractant receptors synergistically regulate leukocyte migration into lymphoid tissues and sites of inflammation, but little is known about how these families of receptors modulate each other's function. In this study, L-selectin was found to be phosphorylated in lymphoblastoid cell lines, and phosphorylation was enhanced by phorbol ester (phorbol 12-myristate 13-acetate (PMA)) treatment. Interactions between L-selectin and chemoattractant receptors were therefore examined using transfected rat basophilic leukemia cell lines (RBL-2H3) that expressed human L-selectin along with human leukocyte chemoattractant receptors. L-selectin was rapidly phosphorylated in cells treated with chemoattractants, thrombin, IgE receptor agonists, or PMA. Pertussis toxin or the protein kinase C inhibitor, staurosporine, completely blocked chemoattractant receptor-induced phosphorylation of L-selectin. PMA-induced phosphorylation was on serine residues within the cytoplasmic tail of L-selectin that have been well conserved during recent evolution. Although L-selectin phosphorylation was not essential for basal levels of adhesion through L-selectin in transformed cell lines, the rapid increase in ligand binding activity of L-selectin that occurs following leukocyte activation was blocked by staurosporine. These results demonstrate that L-selectin can be phosphorylated following engagement of chemoattractant receptors and suggest that this may be a physiologically relevant mechanism for the synergistic regulation of these receptors during leukocyte migration.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 23, 1997

Volume

272

Issue

21

Start / End Page

13961 / 13965

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Serine
  • Receptors, Peptide
  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Rats
  • Protein Kinase C
  • Phosphorylation
  • N-Formylmethionine Leucyl-Phenylalanine
 

Citation

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MLA
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Haribabu, B., Steeber, D. A., Ali, H., Richardson, R. M., Snyderman, R., & Tedder, T. F. (1997). Chemoattractant receptor-induced phosphorylation of L-selectin. J Biol Chem, 272(21), 13961–13965. https://doi.org/10.1074/jbc.272.21.13961
Haribabu, B., D. A. Steeber, H. Ali, R. M. Richardson, R. Snyderman, and T. F. Tedder. “Chemoattractant receptor-induced phosphorylation of L-selectin.J Biol Chem 272, no. 21 (May 23, 1997): 13961–65. https://doi.org/10.1074/jbc.272.21.13961.
Haribabu B, Steeber DA, Ali H, Richardson RM, Snyderman R, Tedder TF. Chemoattractant receptor-induced phosphorylation of L-selectin. J Biol Chem. 1997 May 23;272(21):13961–5.
Haribabu, B., et al. “Chemoattractant receptor-induced phosphorylation of L-selectin.J Biol Chem, vol. 272, no. 21, May 1997, pp. 13961–65. Pubmed, doi:10.1074/jbc.272.21.13961.
Haribabu B, Steeber DA, Ali H, Richardson RM, Snyderman R, Tedder TF. Chemoattractant receptor-induced phosphorylation of L-selectin. J Biol Chem. 1997 May 23;272(21):13961–13965.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 23, 1997

Volume

272

Issue

21

Start / End Page

13961 / 13965

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Serine
  • Receptors, Peptide
  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Rats
  • Protein Kinase C
  • Phosphorylation
  • N-Formylmethionine Leucyl-Phenylalanine