Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation.

Published

Journal Article

We have examined the expression of a cell surface antigen by B lineage cells in human fetuses, newborns and adults using a newly produced monoclonal antibody, HB-7. The HB-7 antigen was found to be a protease sensitive 45,000 MW molecule that appeared to be the same molecule recognized by the OKT-10 antibody. The HB-7 reactive molecule was expressed by all fetal pre-B and B cells, and 50% of newborn blood and adult bone marrow B cells. In contrast, only a small minority of B cells (2-12%) from blood, spleen and tonsil of adults were weakly HB-7+. The pokeweed mitogen-responsive B cell precursors of plasma cells were also HB-7-, but the HB-7 antigen was re-expressed during the plasma cell stage. We conclude that this antigen is unique among known B cell differentiation antigens in its intermittent pattern of expression during B cell development. The reactivity of the HB-7 antibody with immature, but not mature, B cells makes it well suited for studies of B cell ontogeny.

Full Text

Duke Authors

Cited Authors

  • Tedder, TF; Clement, LT; Cooper, MD

Published Date

  • September 1, 1984

Published In

Volume / Issue

  • 24 / 3

Start / End Page

  • 140 - 149

PubMed ID

  • 6440308

Pubmed Central ID

  • 6440308

International Standard Serial Number (ISSN)

  • 0001-2815

Digital Object Identifier (DOI)

  • 10.1111/j.1399-0039.1984.tb02118.x

Language

  • eng

Conference Location

  • England