Transcription factor NF-kappaB regulates inducible CD83 gene expression in activated T lymphocytes.

Published

Journal Article

The immunoglobulin superfamily member CD83 is expressed on the surface of mature dendritic cells that present processed antigens to T lymphocytes. In addition, T cells acquire CD83 expression following mitogenic stimulation in vitro. Here we report two lines of evidence demonstrating that this inducible lymphocyte response is genetically programmed by transcription factor NF-kappaB and contingent upon proteolytic breakdown of its cytoplasmic inhibitor IkappaBalpha. First, signal-dependent induction of CD83 mRNA expression is blocked in both transformed and primary T cells harboring a degradation-resistant mutant of IkappaBalpha that constitutively represses NF-kappaB. Second, as revealed in gel retardation assays, the IkappaBalpha constitutive repressor prevents the inducible interaction of NF-kappaB with consensus recognition sites identified in the CD83 promoter. Given that IkappaBalpha is functionally coupled to the T-cell antigen receptor, these findings suggest that the downstream transcription unit for CD83 is triggered by NF-kappaB during an adaptive immune response.

Full Text

Duke Authors

Cited Authors

  • McKinsey, TA; Chu, Z; Tedder, TF; Ballard, DW

Published Date

  • August 2000

Published In

Volume / Issue

  • 37 / 12-13

Start / End Page

  • 783 - 788

PubMed ID

  • 11275263

Pubmed Central ID

  • 11275263

International Standard Serial Number (ISSN)

  • 0161-5890

Digital Object Identifier (DOI)

  • 10.1016/s0161-5890(00)00099-7

Language

  • eng

Conference Location

  • England