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Tonopen measurement of intraocular pressure in mice.

Publication ,  Journal Article
Reitsamer, HA; Kiel, JW; Harrison, JM; Ransom, NL; McKinnon, SJ
Published in: Exp Eye Res
April 2004

PURPOSE: To standardize a method of non-invasive measurement of intraocular pressure (IOP) in mice. METHODS: Cannulated-eye study: IOP was measured simultaneously with a Tonopen and by direct cannulation of the vitreous compartment while pressure was manipulated in steps between 10 and 45 mmHg by a saline reservoir via a second vitreal cannula (five mice, one rat). Non-cannulated-eye study: Tonopen and servo-null measurements were performed in independent groups (48 mice) to verify Tonopen measurements in non-cannulated-eyes. Topical brimonidine (0.15%) was used to decrease IOP. RESULTS: In the rat, there was a similar relationship between Tonopen readings and direct measurements via cannulation of the eye as previously reported. Although readings from mice eyes were higher in variability than those obtained from the rat, the measurements were reproducible and the correlation between the invasive and the non-invasive methods was good (r = 0.97). The IOP lowering effect of brimonidine was detected with Tonopen as well as servo-null measurements (p < 0.001) and the results with both techniques were similar. CONCLUSION: The Tonopen can be used for rapid and reproducible measurements of IOP in mice. The method is easy to apply and can provide a useful means for IOP measurement in mouse models of induced ocular hypertension, in knock-out and transgenic mice, or in pharmacological studies.

Duke Scholars

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

April 2004

Volume

78

Issue

4

Start / End Page

799 / 804

Location

England

Related Subject Headings

  • Reproducibility of Results
  • Rats, Inbred BN
  • Rats
  • Quinoxalines
  • Ophthalmology & Optometry
  • Models, Animal
  • Mice, Inbred C57BL
  • Mice
  • Manometry
  • Intraocular Pressure
 

Citation

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ICMJE
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Reitsamer, H. A., Kiel, J. W., Harrison, J. M., Ransom, N. L., & McKinnon, S. J. (2004). Tonopen measurement of intraocular pressure in mice. Exp Eye Res, 78(4), 799–804. https://doi.org/10.1016/j.exer.2003.11.018
Reitsamer, Herbert A., Jeffrey W. Kiel, Joseph M. Harrison, Nancy L. Ransom, and Stuart J. McKinnon. “Tonopen measurement of intraocular pressure in mice.Exp Eye Res 78, no. 4 (April 2004): 799–804. https://doi.org/10.1016/j.exer.2003.11.018.
Reitsamer HA, Kiel JW, Harrison JM, Ransom NL, McKinnon SJ. Tonopen measurement of intraocular pressure in mice. Exp Eye Res. 2004 Apr;78(4):799–804.
Reitsamer, Herbert A., et al. “Tonopen measurement of intraocular pressure in mice.Exp Eye Res, vol. 78, no. 4, Apr. 2004, pp. 799–804. Pubmed, doi:10.1016/j.exer.2003.11.018.
Reitsamer HA, Kiel JW, Harrison JM, Ransom NL, McKinnon SJ. Tonopen measurement of intraocular pressure in mice. Exp Eye Res. 2004 Apr;78(4):799–804.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

April 2004

Volume

78

Issue

4

Start / End Page

799 / 804

Location

England

Related Subject Headings

  • Reproducibility of Results
  • Rats, Inbred BN
  • Rats
  • Quinoxalines
  • Ophthalmology & Optometry
  • Models, Animal
  • Mice, Inbred C57BL
  • Mice
  • Manometry
  • Intraocular Pressure