In-vivo response to free electron laser incision of the rabbit cornea.

Journal Article (Journal Article)

BACKGROUND AND OBJECTIVE: We analyzed the in vivo ocular response to corneal incisions made by Medical Free Electron Laser (MFEL) as a function of scan rate and incision depth. Additionally, we compared biomicroscopy, optical coherence tomography (OCT), and light microscopy as ocular response diagnostic tools. STUDY DESIGN/MATERIALS AND METHODS: Rabbit corneas were incised with pulsed MFEL radiation at 2.94 microm wavelength, scalpel incisions or focal cautery treatment were used as controls. The MFEL beam scan rate ranged from 0.2 to 1.0 mm/second. Ocular effects were monitored for 2 hours postoperatively using OCT and slit lamp examination. Ocular tissue was fixed for light microscopic examination. RESULTS: Anterior chamber fibrin formation correlated with MFEL incision depth. Slower scan rates resulted in deeper incisions and greater fibrin formation. OCT was better than slit lamp biomicroscopy at identifying fibrin attachments. OCT and light microscopy proved to be excellent companion technologies. CONCLUSIONS: Deep corneal incisions in the rabbit produced by the MFEL resulted in fibrin formation in the anterior chamber.

Full Text

Duke Authors

Cited Authors

  • Toth, CA; Chiu, EK; Winter, KP; McCall, MN; Bailey, SF; Harkrider, CJ; Hauger, S

Published Date

  • 2001

Published In

Volume / Issue

  • 29 / 1

Start / End Page

  • 44 - 52

PubMed ID

  • 11500862

International Standard Serial Number (ISSN)

  • 0196-8092

Digital Object Identifier (DOI)

  • 10.1002/lsm.1085


  • eng

Conference Location

  • United States