Intraocular injection of recombinant hirudin to prevent experimental postoperative fibrin.

Published

Journal Article

PURPOSE: To test the efficacy of a single intraocular injection of various doses of recombinant desulphatohirudin variant 1 (Revasc) in a rabbit model after lensectomy-vitrectomy surgery. METHODS: Standard fragmatome lensectomies and core vitrectomies were performed on Dutch Belted rabbits. In a masked fashion, 17 control eyes received single intraocular injections of lactated Ringer's solution, and the treated eyes, with eight eyes per treatment group, received single intraocular injections of hirudin at doses of 0.02 microgram, 2 micrograms, 10 micrograms, 50 micrograms, or 200 micrograms. RESULTS: Intraocular injections of hirudin were effective in preventing postoperative fibrin formation at doses of 2 micrograms. There was no notable intraoperative bleeding. With an injection of 2.0 micrograms hirudin there was no hemorrhage at any time after surgery; with an injection of 200 micrograms hirudin, however, there was notable bleeding. CONCLUSION: Hirudin is effective in the prevention of postoperative fibrin formation with a single intraocular injection of 2.0 micrograms after lensectomy-vitrectomy surgery in the rabbit model. There was no bleeding at the effective dose of 2.0 micrograms; at 100 times the effective dose, however, there was significant postoperative bleeding.

Full Text

Duke Authors

Cited Authors

  • Toth, CA; Nasir, MA; Ho, CL; Mittra, RA

Published Date

  • January 1997

Published In

Volume / Issue

  • 17 / 4

Start / End Page

  • 315 - 320

PubMed ID

  • 9279948

Pubmed Central ID

  • 9279948

Electronic International Standard Serial Number (EISSN)

  • 1539-2864

International Standard Serial Number (ISSN)

  • 0275-004X

Digital Object Identifier (DOI)

  • 10.1097/00006982-199707000-00007

Language

  • eng