Quantitative assessment of growth stimulating activity of the vitreous during PVR.
Journal Article
This report postulates that the activity of cellular proliferation along the surface of the retina during proliferative vitreoretinopathy (PVR) is reflected in the aggregate effect of proliferation-inducing growth factors in the vitreous. A method for quantifying the "net" proliferation-inducing capacity of an individual vitreous sample was developed and this assay was used to evaluate the vitreous proliferative activity in a model for experimental PVR. Vitreous was aspirated sequentially after onset of fibroblast-induced PVR in a rabbit model. A simple bioassay for the "aggregate" stimulating activity was developed and each sample was assigned a quantitative value in terms of "proliferation units" (PU). Experimental eyes demonstrated a wide range of stimulating activity (0-1765 PU), but control eyes showed uniformly low levels of activity (0-337 PU). Experimental eyes that ultimately developed retinal detachment displayed higher levels of proliferative activity than did those eyes destined to remain attached. The differences were statistically different by day 3, prior to the onset of clinical retinal changes. We conclude that quantification of vitreous proliferation-stimulating activity is possible and that this method might be useful for screening eyes at high risk for the development of recurrent retinal detachment from PVR.
Full Text
Duke Authors
Cited Authors
- Yang, CM; Cousins, SW
Published Date
- July 1992
Published In
Volume / Issue
- 33 / 8
Start / End Page
- 2436 - 2442
PubMed ID
- 1634341
Pubmed Central ID
- 1634341
International Standard Serial Number (ISSN)
- 0146-0404
Language
- eng
Conference Location
- United States