Perforin knockout mice, but not mice with MAIDS, show protection against experimental cytomegalovirus retinitis after adoptive transfer of immune cells with a functional perforin cytotoxic pathway.
Journal Article (Journal Article)
Adoptive transfer studies were performed to test the hypothesis that the perforin cytotoxic pathway is more important than the Fas/FasL cytotoxic pathway in protection against experimental murine cytomegalovirus (MCMV) retinitis. Splenic immune cells from donor MCMV-immunized normal mice or gld mice deficient in Fas/FasL-mediated cytotoxicity significantly reduced the frequency and severity of MCMV retinitis following subretinal MCMV challenge when transferred into recipient PKO mice deficient in perforin-mediated cytotoxicity. In sharp contrast, splenic cells from donor MCMV-immunized PKO mice failed to provide protection against MCMV retinitis when transferred into recipient PKO mice. Protection was not achieved, however, in recipient mice with retrovirus-induced immunodeficiency (MAIDS), even when splenic cells originated from MCMV-immunized normal mice.
Full Text
Duke Authors
Cited Authors
- Dix, RD; Ekworomadu, CO; Hernandez, E; Cousins, SW
Published Date
- November 2004
Published In
Volume / Issue
- 149 / 11
Start / End Page
- 2235 - 2244
PubMed ID
- 15503209
International Standard Serial Number (ISSN)
- 0304-8608
Digital Object Identifier (DOI)
- 10.1007/s00705-004-0370-3
Language
- eng
Conference Location
- Austria