Perforin knockout mice, but not mice with MAIDS, show protection against experimental cytomegalovirus retinitis after adoptive transfer of immune cells with a functional perforin cytotoxic pathway.

Journal Article (Journal Article)

Adoptive transfer studies were performed to test the hypothesis that the perforin cytotoxic pathway is more important than the Fas/FasL cytotoxic pathway in protection against experimental murine cytomegalovirus (MCMV) retinitis. Splenic immune cells from donor MCMV-immunized normal mice or gld mice deficient in Fas/FasL-mediated cytotoxicity significantly reduced the frequency and severity of MCMV retinitis following subretinal MCMV challenge when transferred into recipient PKO mice deficient in perforin-mediated cytotoxicity. In sharp contrast, splenic cells from donor MCMV-immunized PKO mice failed to provide protection against MCMV retinitis when transferred into recipient PKO mice. Protection was not achieved, however, in recipient mice with retrovirus-induced immunodeficiency (MAIDS), even when splenic cells originated from MCMV-immunized normal mice.

Full Text

Duke Authors

Cited Authors

  • Dix, RD; Ekworomadu, CO; Hernandez, E; Cousins, SW

Published Date

  • November 2004

Published In

Volume / Issue

  • 149 / 11

Start / End Page

  • 2235 - 2244

PubMed ID

  • 15503209

International Standard Serial Number (ISSN)

  • 0304-8608

Digital Object Identifier (DOI)

  • 10.1007/s00705-004-0370-3


  • eng

Conference Location

  • Austria