Monocyte activation in patients with age-related macular degeneration: a biomarker of risk for choroidal neovascularization?

Journal Article (Journal Article)

OBJECTIVE: To evaluate the activation state of macrophage function in patients with age-related macular degeneration (AMD) by quantifying the production of the proinflammatory and angiogenic factor tumor necrosis factor alpha (TNF-alpha) and by correlating its expression with dry and wet AMD. METHODS: Circulating monocytes were obtained from the blood of patients with AMD or age-matched control subjects by gradient centrifugation. The monocytes were then analyzed for either TNF-alpha release from cultured macrophages in response to retinal pigment epithelium-derived blebs and cytokines or TNF-alpha messenger RNA content by reverse transcriptase-polymerase chain reaction. RESULTS: In human monocytes obtained from controls and AMD patients, TNF-alpha was expressed by freshly isolated monocytes and produced by macrophages in culture after stimulation with retinal pigment epithelium-derived blebs. However, wide variability in TNF-alpha expression was observed among different patients. Patients with monocytes that expressed the greatest amount of TNF-alpha demonstrated higher prevalence of choroidal neovascularization. CONCLUSIONS: Both controls and AMD patients vary in the activation state (defined as TNF-alpha expression) of circulating monocytes. Partially active monocytes, defined as high TNF-alpha expression, may be a biomarker to identify patients at risk for formation of choroidal neovascularization. CLINICAL RELEVANCE: Early diagnostic testing may prove useful to detect those patients who will progress to the more severe complications of the disease.

Full Text

Duke Authors

Cited Authors

  • Cousins, SW; Espinosa-Heidmann, DG; Csaky, KG

Published Date

  • July 1, 2004

Published In

Volume / Issue

  • 122 / 7

Start / End Page

  • 1013 - 1018

PubMed ID

  • 15249366

International Standard Serial Number (ISSN)

  • 0003-9950

Digital Object Identifier (DOI)

  • 10.1001/archopht.122.7.1013


  • eng

Conference Location

  • United States