Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.

Published

Journal Article

Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed a rapid semiquantitative assay for infectious MCMV based on expression of beta-galactosidase using a LacZ-expressing recombinant MCMV. Simultaneous use of this assay in combination with a modified cell-mediated cytotoxicity assay revealed that cytotoxicity of MCMV-infected target cells mediated by MCMV-immune splenic cells does not lead to inactivation of intracellular infectious virus.

Full Text

Duke Authors

Cited Authors

  • Dix, RD; Cousins, SW

Published Date

  • April 2005

Published In

Volume / Issue

  • 150 / 4

Start / End Page

  • 797 - 803

PubMed ID

  • 15614430

Pubmed Central ID

  • 15614430

International Standard Serial Number (ISSN)

  • 0304-8608

Digital Object Identifier (DOI)

  • 10.1007/s00705-004-0459-8

Language

  • eng

Conference Location

  • Austria