Skip to main content

T cell depletion increases susceptibility to murine cytomegalovirus retinitis.

Publication ,  Journal Article
Atherton, SS; Newell, CK; Kanter, MY; Cousins, SW
Published in: Invest Ophthalmol Vis Sci
November 1992

To study the effect of immunosuppression on the development of murine cytomegalovirus (MCMV) retinitis, BALB/c mice were immunosuppressed with methylprednisolone (a corticosteroid) and/or with antibodies against CD4+ and CD8+ T cells and inoculated with low-dose MCMV (5 x 10(2) plaque-forming units) by the supraciliary route. Nonimmunosuppressed mice inoculated with low-dose MCMV by the supraciliary route did not develop necrotizing retinitis. By contrast, 78-100% of immunosuppressed mice developed retinitis after inoculation of low-dose MCMV. To study the effect of depletion of individual T cell subsets, mice were depleted of either CD4+ or CD8+ T cells and inoculated with low-dose MCMV by the supraciliary route. The frequency of retinitis in CD4-depleted mice (30%) was not significantly different from that of nonimmunosuppressed control mice (0%). The frequency of retinitis in the CD8-depleted group (80%) was similar to that observed in mice immunosuppressed with corticosteroid alone (90.9%), with antibodies to both T cell subsets (100%), or with steroid and both T cell subset antibodies (100%). These results support the conclusion that the CD8+ T cell subset is responsible for control of ocular MCMV infection. Furthermore, these results suggest that the CD8+ T cell subset may be important in preventing ocular CMV infection in immunosuppressed patients.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

November 1992

Volume

33

Issue

12

Start / End Page

3353 / 3360

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Retinitis
  • Ophthalmology & Optometry
  • Mice, Inbred BALB C
  • Mice
  • Lymphocyte Depletion
  • Injections
  • Immunosuppression Therapy
  • Disease Susceptibility
  • Cytomegalovirus Infections
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Atherton, S. S., Newell, C. K., Kanter, M. Y., & Cousins, S. W. (1992). T cell depletion increases susceptibility to murine cytomegalovirus retinitis. Invest Ophthalmol Vis Sci, 33(12), 3353–3360.
Atherton, S. S., C. K. Newell, M. Y. Kanter, and S. W. Cousins. “T cell depletion increases susceptibility to murine cytomegalovirus retinitis.Invest Ophthalmol Vis Sci 33, no. 12 (November 1992): 3353–60.
Atherton SS, Newell CK, Kanter MY, Cousins SW. T cell depletion increases susceptibility to murine cytomegalovirus retinitis. Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3353–60.
Atherton, S. S., et al. “T cell depletion increases susceptibility to murine cytomegalovirus retinitis.Invest Ophthalmol Vis Sci, vol. 33, no. 12, Nov. 1992, pp. 3353–60.
Atherton SS, Newell CK, Kanter MY, Cousins SW. T cell depletion increases susceptibility to murine cytomegalovirus retinitis. Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3353–3360.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

November 1992

Volume

33

Issue

12

Start / End Page

3353 / 3360

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Retinitis
  • Ophthalmology & Optometry
  • Mice, Inbred BALB C
  • Mice
  • Lymphocyte Depletion
  • Injections
  • Immunosuppression Therapy
  • Disease Susceptibility
  • Cytomegalovirus Infections