Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.
Journal Article (Journal Article)
Proteoliposomes were reconstituted by detergent dialysis of a sodium cholate extract of inverted vesicles derived from Escherichia coli plasma membrane. The translocation of precursor proteins into reconstituted vesicles occurred at high efficiency and was SecB dependent. The protein composition of the reconstituted vesicles differed markedly from that of native vesicles. Immunoblot analysis of the sodium cholate extract and of the reconstituted vesicles indicated that PrlA (SecY) protein remained largely unsolubilized under the described conditions and was virtually absent from the reconstituted vesicles, suggesting that PrlA may not be required for in vitro translocation.
Full Text
Duke Authors
Cited Authors
- Watanabe, M; Nicchitta, CV; Blobel, G
Published Date
- March 1990
Published In
Volume / Issue
- 87 / 5
Start / End Page
- 1960 - 1964
PubMed ID
- 2408048
Pubmed Central ID
- PMC53604
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.87.5.1960
Language
- eng
Conference Location
- United States