High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts.

Journal Article (Journal Article)

A method for the separation and quantification of the levels of alanopine and strombine in neutralized, perchloric acid extracts of tissues of marine invertebrates is presented. The method is based on high-performance liquid chromatographic (HPLC) separation, postcolumn derivatization using o-phthaldialdehyde and sodium hypochlorite, and subsequent fluorometric detection. Isocratic separation results in the rapid elution of alanopine and strombine, with elution times of 4.7 and 5.4 min, respectively. The sensitivity of this method is in the range 50-250 pmol. However, the fluorometric detection approach provides the capability for even greater sensitivity.

Full Text

Duke Authors

Cited Authors

  • Fiore, GB; Nicchitta, CV; Ellington, WR

Published Date

  • June 1984

Published In

Volume / Issue

  • 139 / 2

Start / End Page

  • 413 - 417

PubMed ID

  • 6206747

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(84)90027-7


  • eng

Conference Location

  • United States