High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts.
Publication
, Journal Article
Fiore, GB; Nicchitta, CV; Ellington, WR
Published in: Anal Biochem
June 1984
A method for the separation and quantification of the levels of alanopine and strombine in neutralized, perchloric acid extracts of tissues of marine invertebrates is presented. The method is based on high-performance liquid chromatographic (HPLC) separation, postcolumn derivatization using o-phthaldialdehyde and sodium hypochlorite, and subsequent fluorometric detection. Isocratic separation results in the rapid elution of alanopine and strombine, with elution times of 4.7 and 5.4 min, respectively. The sensitivity of this method is in the range 50-250 pmol. However, the fluorometric detection approach provides the capability for even greater sensitivity.
Duke Scholars
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
June 1984
Volume
139
Issue
2
Start / End Page
413 / 417
Location
United States
Related Subject Headings
- Tissue Extracts
- Spectrometry, Fluorescence
- Sarcosine
- Ostreidae
- Muscles
- Mollusca
- Glycine
- Chromatography, High Pressure Liquid
- Bivalvia
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Fiore, G. B., Nicchitta, C. V., & Ellington, W. R. (1984). High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts. Anal Biochem, 139(2), 413–417. https://doi.org/10.1016/0003-2697(84)90027-7
Fiore, G. B., C. V. Nicchitta, and W. R. Ellington. “High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts.” Anal Biochem 139, no. 2 (June 1984): 413–17. https://doi.org/10.1016/0003-2697(84)90027-7.
Fiore GB, Nicchitta CV, Ellington WR. High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts. Anal Biochem. 1984 Jun;139(2):413–7.
Fiore, G. B., et al. “High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts.” Anal Biochem, vol. 139, no. 2, June 1984, pp. 413–17. Pubmed, doi:10.1016/0003-2697(84)90027-7.
Fiore GB, Nicchitta CV, Ellington WR. High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts. Anal Biochem. 1984 Jun;139(2):413–417.
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
June 1984
Volume
139
Issue
2
Start / End Page
413 / 417
Location
United States
Related Subject Headings
- Tissue Extracts
- Spectrometry, Fluorescence
- Sarcosine
- Ostreidae
- Muscles
- Mollusca
- Glycine
- Chromatography, High Pressure Liquid
- Bivalvia
- Biochemistry & Molecular Biology