Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitro.


Journal Article

Regulation of humoral responses involves multiple cell types including the requirements for cognate interactions between T and B cells to drive CD40-dependent responses to T-dependent antigens. A third cell type has also been shown to play an essential role, the dendritic cell (DC). We demonstrate that bovine peripheral blood-derived (PB)-DC are similar in function to features described for human interstitial DC including the production of signature type 2 cytokines [interleukin (IL)-13, IL-10]. PB-DC express moderate-to-high costimulatory molecule expression, and major histocompatibility complex class II is negative for CD14 expression and has low or no expression of CD11c. Consistent with the interstitial phenotype is the ability of PB-DC to influence B cell activation and differentiation via direct expression of CD40L and type 2 cytokines. Collectively, these results suggest that direct B cell-DC interactions may promote an immunoglobulin-isotype expression pattern consistent with type 2 responses, independent of direct T cell involvement.

Full Text

Cited Authors

  • Bajer, AA; Garcia-Tapia, D; Jordan, KR; Haas, KM; Werling, D; Howard, CJ; Estes, DM

Published Date

  • January 2003

Published In

Volume / Issue

  • 73 / 1

Start / End Page

  • 100 - 106

PubMed ID

  • 12525567

Pubmed Central ID

  • 12525567

Electronic International Standard Serial Number (EISSN)

  • 1938-3673

International Standard Serial Number (ISSN)

  • 0741-5400

Digital Object Identifier (DOI)

  • 10.1189/jlb.0302128


  • eng