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Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine.

Publication ,  Journal Article
Cho, MC; Rao, M; Koch, WJ; Thomas, SA; Palmiter, RD; Rockman, HA
Published in: Circulation
May 25, 1999

BACKGROUND: Elevated circulating norepinephrine (NE) has been implicated in causing the profound beta-adrenergic receptor (betaAR) downregulation and receptor uncoupling that are characteristic of end-stage human dilated cardiomyopathy, a process mediated in part by increased levels of beta-adrenergic receptor kinase (betaARK1). To explore whether chronic sustained NE stimulation is a primary stimulus that promotes deterioration in cardiac signaling, we characterized a gene-targeted mouse in which activation of the sympathetic nervous system cannot lead to an elevation in plasma NE and epinephrine. METHODS AND RESULTS: Gene-targeted mice that lack dopamine beta-hydroxylase (dbh-/-), the enzyme needed to convert dopamine to NE, were created by homologous recombination. In vivo contractile response to the beta1AR agonist dobutamine, measured by a high-fidelity left ventricular micromanometer, was enhanced in mice lacking the dbh gene. In unloaded adult myocytes isolated from dbh-/- mice, basal contractility was significantly increased compared with control cells. Furthermore, the increase in betaAR responsiveness and enhanced cellular contractility were associated with a significant reduction in activity and protein level of betaARK1 and increased high-affinity agonist binding without changes in betaAR density or G-protein levels. CONCLUSIONS: Mice that lack the ability to generate NE or epinephrine show increased contractility associated primarily with a decrease in the level of betaARK1 protein and kinase activity. This animal model will be valuable in testing whether NE is required for the pathogenesis of heart failure through mating strategies that cross the dbh-/- mouse into genetically engineered models of heart failure.

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Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

May 25, 1999

Volume

99

Issue

20

Start / End Page

2702 / 2707

Location

United States

Related Subject Headings

  • beta-Adrenergic Receptor Kinases
  • Sarcolemma
  • Receptors, Adrenergic, beta
  • Norepinephrine
  • Myocardial Contraction
  • Mice, Mutant Strains
  • Mice
  • Male
  • Hemodynamics
  • Gene Targeting
 

Citation

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Cho, M. C., Rao, M., Koch, W. J., Thomas, S. A., Palmiter, R. D., & Rockman, H. A. (1999). Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine. Circulation, 99(20), 2702–2707. https://doi.org/10.1161/01.cir.99.20.2702
Cho, M. C., M. Rao, W. J. Koch, S. A. Thomas, R. D. Palmiter, and H. A. Rockman. “Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine.Circulation 99, no. 20 (May 25, 1999): 2702–7. https://doi.org/10.1161/01.cir.99.20.2702.
Cho MC, Rao M, Koch WJ, Thomas SA, Palmiter RD, Rockman HA. Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine. Circulation. 1999 May 25;99(20):2702–7.
Cho, M. C., et al. “Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine.Circulation, vol. 99, no. 20, May 1999, pp. 2702–07. Pubmed, doi:10.1161/01.cir.99.20.2702.
Cho MC, Rao M, Koch WJ, Thomas SA, Palmiter RD, Rockman HA. Enhanced contractility and decreased beta-adrenergic receptor kinase-1 in mice lacking endogenous norepinephrine and epinephrine. Circulation. 1999 May 25;99(20):2702–2707.

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

May 25, 1999

Volume

99

Issue

20

Start / End Page

2702 / 2707

Location

United States

Related Subject Headings

  • beta-Adrenergic Receptor Kinases
  • Sarcolemma
  • Receptors, Adrenergic, beta
  • Norepinephrine
  • Myocardial Contraction
  • Mice, Mutant Strains
  • Mice
  • Male
  • Hemodynamics
  • Gene Targeting