Alteration and restoration of K+ channel function by deletions at the N- and C-termini.

Journal Article (Journal Article)

Voltage-dependent ion channels are thought to consist of a highly conserved repeated core of six transmembrane segments, flanked by more variable cytoplasmic domains. Significant functional differences exist among related types of K+ channels. These differences have been attributed to the variable domains, most prominently the N- and C-termini. We have therefore investigated the functional importance of both termini for the delayed rectifier K+ channel from rat brain encoded by the drk1 gene. This channel has an unusually long C-terminus. Deletions in either terminus affected both activation and inactivation, in some cases profoundly. Unexpectedly, more extensive deletions in both termini restored gating. We could therefore define a core region only slightly longer than the six transmembrane segments that is sufficient for the formation of channels with the kinetics of a delayed rectifier.

Full Text

Duke Authors

Cited Authors

  • VanDongen, AM; Frech, GC; Drewe, JA; Joho, RH; Brown, AM

Published Date

  • October 1990

Published In

Volume / Issue

  • 5 / 4

Start / End Page

  • 433 - 443

PubMed ID

  • 2206531

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/0896-6273(90)90082-q


  • eng

Conference Location

  • United States