Toxin and kinetic profile of rat brain type III sodium channels expressed in Xenopus oocytes.

Published

Journal Article

Sodium (Na+) channels are members of a multigene family and are responsible for generation and propagation of the action potential in excitable cells. We have assembled, in a transcription-competent vector, a full-length cDNA clone encoding the rat brain type III Na+ channel. Xenopus oocytes microinjected with in vitro synthesized mRNA expressed functional rat brain Na+ channels from such 'cloned' RNA transcripts. We found that type III Na+ currents in whole cell microelectrode voltage clamp and in cell-attached patch recordings decayed much more slowly than any other reported Na+ current. In addition, we saw typical and additive effects of alpha- and beta-scorpion toxins, suggesting that the Na+ channel alpha-subunit itself contains functional and distinct toxin binding sites.

Full Text

Duke Authors

Cited Authors

  • Joho, RH; Moorman, JR; VanDongen, AM; Kirsch, GE; Silberberg, H; Schuster, G; Brown, AM

Published Date

  • February 1990

Published In

Volume / Issue

  • 7 / 2

Start / End Page

  • 105 - 113

PubMed ID

  • 2160038

Pubmed Central ID

  • 2160038

International Standard Serial Number (ISSN)

  • 0169-328X

Digital Object Identifier (DOI)

  • 10.1016/0169-328x(90)90087-t

Language

  • eng