Toxin and kinetic profile of rat brain type III sodium channels expressed in Xenopus oocytes.
Journal Article (Journal Article)
Sodium (Na+) channels are members of a multigene family and are responsible for generation and propagation of the action potential in excitable cells. We have assembled, in a transcription-competent vector, a full-length cDNA clone encoding the rat brain type III Na+ channel. Xenopus oocytes microinjected with in vitro synthesized mRNA expressed functional rat brain Na+ channels from such 'cloned' RNA transcripts. We found that type III Na+ currents in whole cell microelectrode voltage clamp and in cell-attached patch recordings decayed much more slowly than any other reported Na+ current. In addition, we saw typical and additive effects of alpha- and beta-scorpion toxins, suggesting that the Na+ channel alpha-subunit itself contains functional and distinct toxin binding sites.
Full Text
Duke Authors
Cited Authors
- Joho, RH; Moorman, JR; VanDongen, AM; Kirsch, GE; Silberberg, H; Schuster, G; Brown, AM
Published Date
- February 1990
Published In
Volume / Issue
- 7 / 2
Start / End Page
- 105 - 113
PubMed ID
- 2160038
International Standard Serial Number (ISSN)
- 0169-328X
Digital Object Identifier (DOI)
- 10.1016/0169-328x(90)90087-t
Language
- eng
Conference Location
- Netherlands