Isolation of neural stem cells from the postnatal cerebellum.

Published

Journal Article

The cerebellum is critical for motor coordination and cognitive function and is the target of transformation in medulloblastoma, the most common malignant brain tumor in children. Although the development of granule cells, the most abundant neurons in the cerebellum, has been studied in detail, the origins of other cerebellar neurons and glia remain poorly understood. Here we show that the murine postnatal cerebellum contains multipotent neural stem cells (NSCs). These cells can be prospectively isolated based on their expression of the NSC marker prominin-1 (CD133) and their lack of markers of neuronal and glial lineages (lin-). Purified prominin+ lin- cells form self-renewing neurospheres and can differentiate into astrocytes, oligodendrocytes and neurons in vitro. Moreover, they can generate each of these lineages after transplantation into the cerebellum. Identification of cerebellar stem cells has important implications for the understanding of cerebellar development and the origins of medulloblastoma.

Full Text

Cited Authors

  • Lee, A; Kessler, JD; Read, T-A; Kaiser, C; Corbeil, D; Huttner, WB; Johnson, JE; Wechsler-Reya, RJ

Published Date

  • June 2005

Published In

Volume / Issue

  • 8 / 6

Start / End Page

  • 723 - 729

PubMed ID

  • 15908947

Pubmed Central ID

  • 15908947

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

International Standard Serial Number (ISSN)

  • 1097-6256

Digital Object Identifier (DOI)

  • 10.1038/nn1473

Language

  • eng