Effects of cholecystokinin, gastrin, and related peptides on coho salmon gallbladder contraction in vitro.

Published

Journal Article

UNLABELLED: Isometric contractions of coho salmon (Oncorhynchus kisutch) gallbladder longitudinal muscle strips were recorded in response to porcine cholecystokinin (CCK), octapeptide of CCK (OP-CCK), desulfated octapeptide of CCK (ds-OP-CCK), porcine heptadecapeptide gastrins I and II, and pentagastrin. Peak tonic contraction amplitude and peak rhythmic contraction frequency were used to construct concentration-response curves for each peptide. Observed maximal responses to each peptide were not significantly different; therefore, all peptides tested were equal in efficacy. CCK, OP-CCK, and gastrin II were equipotent, and ds-OP-CCK and gastrin I were equipotent. CONCLUSIONS: 1) porcine CCK can stimulate contraction of the coho salmon gallbladder; 2) tonic and rhythmic contractile responses are elicited in coho salmon gallbladder by agonists; 3) coho salmon gallbladder muscle cannot distinguish between agonists that differ in the location of a sulfated tyrosyl residue at position 6 versus position 7 from the C-terminus; and 4) the results of this study are consistent with the hypothesis that coho salmon gallbladder muscle contains a relatively primitive CCK receptor.

Full Text

Duke Authors

Cited Authors

  • Vigna, SR; Gorbman, A

Published Date

  • May 1, 1977

Published In

Volume / Issue

  • 232 / 5

Start / End Page

  • E485 - E491

PubMed ID

  • 860759

Pubmed Central ID

  • 860759

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpendo.1977.232.5.E485

Language

  • eng

Conference Location

  • United States