Capsaicin vanilloid receptor-1 mediates substance P release in experimental pancreatitis.
Published
Journal Article
We examined whether the capsaicin vanilloid receptor-1 (VR1) mediates substance P (SP) release from primary sensory neurons in experimental pancreatitis. Pancreatitis was achieved by 12 hourly injections of caerulein (50 microg/kg ip) in mice. One group received capsazepine (100 micromol/kg sc), a competitive VR1 antagonist, at 4-h intervals. Neurokinin-1 receptor (NK1R) internalization in acinar cells, used as an index of endogenous SP release, was assessed by immunocytochemical quantification of NK1R endocytosis. The severity of pancreatitis was assessed by measurements of serum amylase, pancreatic myeloperoxidase (MPO) activity, and histological grading. Caerulein administration caused significant elevations in serum amylase and pancreatic MPO activity, produced histological evidence of pancreatitis, and caused a dramatic increase in NK1R endocytosis. Capsazepine treatment significantly reduced the level of NK1R endocytosis, and this was associated with similar reductions in pancreatic MPO activity and histological severity of pancreatitis. These results demonstrate that repeated caerulein stimulation causes experimental pancreatitis that is mediated in part by stimulation of VR1 on primary sensory neurons, resulting in endogenous SP release.
Full Text
Duke Authors
Cited Authors
- Nathan, JD; Patel, AA; McVey, DC; Thomas, JE; Prpic, V; Vigna, SR; Liddle, RA
Published Date
- November 2001
Published In
Volume / Issue
- 281 / 5
Start / End Page
- G1322 - G1328
PubMed ID
- 11668042
Pubmed Central ID
- 11668042
International Standard Serial Number (ISSN)
- 0193-1857
Digital Object Identifier (DOI)
- 10.1152/ajpgi.2001.281.5.G1322
Language
- eng
Conference Location
- United States