Regulation of cholecystokinin secretion by bombesin in STC-1 cells.

Published

Journal Article

Bombesin stimulates cholecystokinin (CCK) secretion, presumably by a direct effect on the intestinal CCK cell. The present objectives were to characterize bombesin-stimulated CCK release and to investigate the role of calcium in CCK secretion in an intestinal CCK-producing cell line (STC-1). Bombesin caused a dose-dependent release of CCK, which was reduced either in the absence of extracellular calcium or by calcium channel blockade, suggesting that influx of calcium is necessary for CCK secretion. Bombesin caused an increase in intracellular calcium concentration ([Ca2+]i) and increased efflux of 45Ca2+ from 45Ca(2+)-loaded cells. Radioligand binding studies and Northern analysis were consistent with the expression of a bombesin receptor. Thus bombesin stimulation of CCK release occurs via binding to a receptor and is dependent on increased [Ca2+]i. We propose that the STC-1 cell line may provide a useful model for studying the regulation of intestinal CCK secretion.

Full Text

Duke Authors

Cited Authors

  • Snow, ND; Prpic, V; Mangel, AW; Sharara, AI; McVey, DC; Hurst, LJ; Vigna, SR; Liddle, RA

Published Date

  • November 1994

Published In

Volume / Issue

  • 267 / 5 Pt 1

Start / End Page

  • G859 - G865

PubMed ID

  • 7977748

Pubmed Central ID

  • 7977748

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpgi.1994.267.5.G859

Language

  • eng

Conference Location

  • United States