Characterization of peptide YY receptors in rabbit colonic mucosa.

Published

Journal Article

Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY > or = neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.

Full Text

Duke Authors

Cited Authors

  • Mannon, PJ; Hernandez, EJ; Mervin, SJ; Vigna, SR; Taylor, IL

Published Date

  • May 1993

Published In

Volume / Issue

  • 14 / 3

Start / End Page

  • 567 - 572

PubMed ID

  • 8392723

Pubmed Central ID

  • 8392723

International Standard Serial Number (ISSN)

  • 0196-9781

Digital Object Identifier (DOI)

  • 10.1016/0196-9781(93)90146-8

Language

  • eng

Conference Location

  • United States