Biological activity of a bombesin-like peptide extracted from the intestine of the ratfish, Hydrolagus colliei.


Journal Article

1. Ratfish (Hydrolagus colliei) intestines were boiled in water to inactivate proteases and then treated with cold 4% trifluoroacetic acid to extract bombesin-like peptides. 2. The extract was fractionated in several steps using reverse-phase and ion exchange HPLC, and bombesin-like immunoreactive peptides were detected by radioimmunoassay using an antiserum specific for the bioactive C-terminal region of bombesin. 3. A highly purified bombesin-like peptide-containing fraction stimulated amylase release in a dose-responsive fashion from rat pancreatic acini; the dose-response curve was parallel to a bombesin standard, and the ratfish peptide stimulated the same maximal rate of amylase secretion as the bombesin standard. 4. A potent, highly selective bombesin receptor antagonist completely abolished the stimulation of amylase release caused by the ratfish peptide, demonstrating the specificity of the response. 5. Estimates of the bombesin-like peptide concentration of this fraction by radioimmunoassay and by bioassay were nearly identical, indicating that ratfish bombesin is very similar biologically and antigenically to frog skin bombesin.

Full Text

Cited Authors

  • Thorndyke, MC; Reeve, JR; Vigna, SR

Published Date

  • 1990

Published In

Volume / Issue

  • 96 / 1

Start / End Page

  • 135 - 140

PubMed ID

  • 1704298

Pubmed Central ID

  • 1704298

International Standard Serial Number (ISSN)

  • 0742-8413

Digital Object Identifier (DOI)

  • 10.1016/0742-8413(90)90058-h


  • eng

Conference Location

  • England