Multiple gastrin-releasing peptide gene-associated peptides are produced by a human small cell lung cancer line.

Published

Journal Article

Products of the gastrin-releasing peptide gene were isolated from culture medium supernatant of a small cell lung cancer line, NCI-H345, by several (high performance liquid chromatography) HPLC steps. The column eluates were monitored by immunoassay and absorbance profiles. Gastrin-releasing peptide was identified in HPLC eluates by a specific radioimmunoassay. Two carboxyl-terminal gastrin-releasing peptide gene-associated peptides were identified by a radioimmunoassay specific for their predicted carboxyl terminus. The amino termini of these two peptides were determined by microsequence analysis. The shorter peptide was revealed to be a fragment of the larger peptide. Expression of an alternate mRNA was shown by isolation and characterization of a novel tetradecapeptide. Amino acid analysis, microsequence analysis, and mass spectral analysis confirmed that the structure was Ser-Leu-Leu-Gln-Val-Leu-Asn-Val-Lys-Glu-Gly-Thr-Pro-Ser. This peptide represents the carboxyl terminus of a peptide resulting from alternate processing of gastrin releasing peptide mRNA. This mRNA contains a 19-base deletion, creating a frame shift. A radioiodinated synthetic analog of this peptide (Tyr-Leu-Val-Asp-Ser-Leu-Leu-Gln-Val-Leu-Asn-Val-Lys-Glu-Gly-Thr-Pro-Ser ) bound specifically to a small cell cancer line with high affinity, suggesting possible biological activity of the isolated peptide.

Full Text

Duke Authors

Cited Authors

  • Reeve, JR; Cuttitta, F; Vigna, SR; Heubner, V; Lee, TD; Shively, JE; Ho, FJ; Fedorko, J; Minna, JD; Walsh, JH

Published Date

  • February 5, 1989

Published In

Volume / Issue

  • 264 / 4

Start / End Page

  • 1928 - 1932

PubMed ID

  • 2536694

Pubmed Central ID

  • 2536694

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States