Biological activity of oxidized and reduced iodinated bombesins.


Journal Article

A method is reported for preparing oxidized and reduced iodinated Tyr4-bombesin. Iodogen was used to iodinate Tyr4-bombesin and the reaction products were separated by reverse-phase HPLC. The peak of oxidized label was then reduced by incubation with 725 mM dithiothreitol at 80 degrees C (pH 8.0) for one hour and the reaction products separated by HPLC as before. The reduced but not oxidized peaks of 125I-Tyr4-bombesin stimulated amylase release from rat pancreatic acini in vitro. We conclude that oxidation of bombesin producing C-terminal methionine sulfoxide destroys the biological activity of the peptide and that this form of oxidation can be reversed.

Full Text

Cited Authors

  • Vigna, SR; Giraud, AS; Reeve, JR; Walsh, JH

Published Date

  • July 1988

Published In

Volume / Issue

  • 9 / 4

Start / End Page

  • 923 - 926

PubMed ID

  • 2465544

Pubmed Central ID

  • 2465544

International Standard Serial Number (ISSN)

  • 0196-9781

Digital Object Identifier (DOI)

  • 10.1016/0196-9781(88)90142-8


  • eng

Conference Location

  • United States