Skip to main content
Journal cover image

Exposure of mice to topical bovine thrombin induces systemic autoimmunity.

Publication ,  Journal Article
Schoenecker, JG; Johnson, RK; Lesher, AP; Day, JD; Love, SD; Hoffman, MR; Ortel, TL; Parker, W; Lawson, JH
Published in: Am J Pathol
November 2001

Bovine thrombin is used as an aid to hemostasis in medical and surgical procedures. At least 500,000 Americans are exposed to this therapeutic annually and reports suggest that exposure is associated with the development of autoreactive antibodies. To determine whether bovine thrombin can induce pathological autoimmunity we exposed nonautoimmune-prone galactose-alpha1-3-galactose-deficient mice to the two bovine thrombin preparations currently approved for use in the United States. We found that, like humans exposed to bovine thrombin, mice developed an immune response against the therapeutic and the xenogeneic carbohydrate galactose-alpha1-3-galactose, and some mice developed autoantibodies against clotting factors. Further, unexpectedly, a single exposure to this therapeutic also induced autoimmunity with features characteristic of systemic lupus erythematosus including antibodies against nuclear antigens, native DNA, double-stranded DNA, and cardiolipin. High levels of these autoantibodies correlated with glomerulonephritis in all mice evaluated. This autoimmune syndrome was detected in mice 15 weeks after a secondary exposure to bovine thrombin and female mice were found to develop the syndrome at a significantly greater frequency than males. Thus, these studies indicate that exposure to bovine thrombin preparations can induce a pathological systemic autoimmune syndrome with lupus-like serology.

Duke Scholars

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

November 2001

Volume

159

Issue

5

Start / End Page

1957 / 1969

Location

United States

Related Subject Headings

  • Time Factors
  • Thrombin
  • Pathology
  • Microscopy, Electron
  • Mice, Knockout
  • Mice
  • Male
  • Galactosyltransferases
  • Female
  • DNA, Single-Stranded
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schoenecker, J. G., Johnson, R. K., Lesher, A. P., Day, J. D., Love, S. D., Hoffman, M. R., … Lawson, J. H. (2001). Exposure of mice to topical bovine thrombin induces systemic autoimmunity. Am J Pathol, 159(5), 1957–1969. https://doi.org/10.1016/S0002-9440(10)63043-X
Schoenecker, J. G., R. K. Johnson, A. P. Lesher, J. D. Day, S. D. Love, M. R. Hoffman, T. L. Ortel, W. Parker, and J. H. Lawson. “Exposure of mice to topical bovine thrombin induces systemic autoimmunity.Am J Pathol 159, no. 5 (November 2001): 1957–69. https://doi.org/10.1016/S0002-9440(10)63043-X.
Schoenecker JG, Johnson RK, Lesher AP, Day JD, Love SD, Hoffman MR, et al. Exposure of mice to topical bovine thrombin induces systemic autoimmunity. Am J Pathol. 2001 Nov;159(5):1957–69.
Schoenecker, J. G., et al. “Exposure of mice to topical bovine thrombin induces systemic autoimmunity.Am J Pathol, vol. 159, no. 5, Nov. 2001, pp. 1957–69. Pubmed, doi:10.1016/S0002-9440(10)63043-X.
Schoenecker JG, Johnson RK, Lesher AP, Day JD, Love SD, Hoffman MR, Ortel TL, Parker W, Lawson JH. Exposure of mice to topical bovine thrombin induces systemic autoimmunity. Am J Pathol. 2001 Nov;159(5):1957–1969.
Journal cover image

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

November 2001

Volume

159

Issue

5

Start / End Page

1957 / 1969

Location

United States

Related Subject Headings

  • Time Factors
  • Thrombin
  • Pathology
  • Microscopy, Electron
  • Mice, Knockout
  • Mice
  • Male
  • Galactosyltransferases
  • Female
  • DNA, Single-Stranded