The effect of factor X level on thrombin generation and the procoagulant effect of activated factor VII in a cell-based model of coagulation.


Journal Article

We used a cell-based, in-vitro model of normal hemostasis and hemophilia to address the question of whether factor (F) X concentration affects the hemostatic response to high-dose activated factor VII (FVIIa). Under conditions designed to mimic normal tissue factor-initiated hemostasis in vivo, we found that only a very small amount of FX -- equivalent to about 3% of the normal plasma level -- was required to support a 'normal' level of thrombin generation. This suggests that, under normal conditions in vivo, the level of FX does not significantly affect hemostatic function. By contrast, in experiments designed to mimic the hemophilic condition, the level of FX had a significant effect on the level of thrombin generated in the presence of high-dose FVIIa. This finding suggests that the plasma level of FX could affect the hemostatic response of hemophilic patients to high-dose FVIIa therapy.

Full Text

Duke Authors

Cited Authors

  • Allen, GA; Monroe, DM; Roberts, HR; Hoffman, M

Published Date

  • April 2000

Published In

Volume / Issue

  • 11 Suppl 1 /

Start / End Page

  • S3 - S7

PubMed ID

  • 10850556

Pubmed Central ID

  • 10850556

International Standard Serial Number (ISSN)

  • 0957-5235

Digital Object Identifier (DOI)

  • 10.1097/00001721-200004001-00002


  • eng

Conference Location

  • England