Analyzing fibrin clot structure using a microplate reader.

Published

Journal Article

Fibrin clot structure studies are often performed using optical methods. For example, the clot's fiber structure can be assessed by measuring light scattering as a function of wavelength. From these measurements, one can calculate the mass/length ratio (mu), a relative measure of fibrin thickness. Fiber thickness has important functional correlates in terms of clot stability and resistance to fibrinolysis. Typically, measurements to calculate mass/length ratios are carried out on high-end spectrophotometers. However, limitations of this instrument include the large sample volume required and the inability to read multiple samples at one time. To circumvent these limitations, a plate-reading spectrophotometer is more commonly used to monitor clot formation; increases in absorbance indicate clot formation, while decreases indicate clot lysis. However, it is unclear whether plate-reading spectrophotometers can be used to quantitatively evaluate fibrin fiber structure. In the current study, we compared spectrophotometric analysis of fibrin gels on single-sample and plate-reading spectrophotometers. Results show that a plate-reading spectrophotometer does not give accurate measurements of the fiber mass/length ratio. However, the plate-reading spectrophotometer can provide a qualitative measure of fiber structure for both purified fibrinogen and plasma. We suggest that plate-reading spectrophotometers can provide a convenient, rapid, and inexpensive means of analyzing fibrin clot structure.

Full Text

Duke Authors

Cited Authors

  • Wolberg, AS; Gabriel, DA; Hoffman, M

Published Date

  • September 2002

Published In

Volume / Issue

  • 13 / 6

Start / End Page

  • 533 - 539

PubMed ID

  • 12192305

Pubmed Central ID

  • 12192305

International Standard Serial Number (ISSN)

  • 0957-5235

Digital Object Identifier (DOI)

  • 10.1097/00001721-200209000-00008

Language

  • eng

Conference Location

  • England