The effects of heparin cofactor II-derived chemotaxins on neutrophil actin conformation and cyclic AMP levels.

Journal Article (Journal Article)

The serine proteinase inhibitor heparin cofactor II (HC) can be cleaved by polymorphonuclear leukocyte (PMN) elastase (LE) to yield potent chemotactic activity for PMN and monocytes. In contrast to the bacterially-derived chemotaxin formyl-Met-Leu-Phe (fMLP), the HC-derived chemotaxin does not stimulate PMN degranulation or oxidative burst activity. We compared the effects of HC-derived chemotaxins to the effects of fMLP on PMN actin conformation and on the cAMP levels. Both the HC chemotaxins and fMLP rapidly induced an increase in F-actin which was similar in magnitude and time-course. However, in contrast to fMLP, HC-derived chemotaxins did not elevate cAMP levels. HC-derived chemotaxins may be useful probes of chemotactic responses, since they do not have the mixed biological activities of fMLP.

Full Text

Duke Authors

Cited Authors

  • Hoffman, M; Faulkner, KA; Iannone, MA; Church, FC

Published Date

  • October 16, 1991

Published In

Volume / Issue

  • 1095 / 1

Start / End Page

  • 78 - 82

PubMed ID

  • 1932129

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0167-4889(91)90047-2


  • eng

Conference Location

  • Netherlands