Platelet heterogeneity: variation in coagulation complexes on platelet subpopulations.

Published

Journal Article

OBJECTIVE: Previous work has shown that platelets stimulated with the combination of thrombin and convulxin, a glycoprotein VI agonist, develop 2 populations with different levels of alpha-granule factor V bound to the platelet surface. To evaluate whether this phenomenon is restricted to alpha-granule components or is a feature that can be generalized to other coagulation factors, we studied the binding of factors V, VIII, IX, and X on the surface of platelets stimulated by convulxin and thrombin. METHODS AND RESULTS: The relative amount of factors V, VIII, IX, and X on the surface of platelets stimulated with thrombin or convulxin plus thrombin was measured using flow cytometry. Simultaneous measurements of factor Xa and thrombin generation were obtained and correlated with the binding of coagulation factors on the platelet surface. The binding of factors V, VIII, IX, and X all increased on a subpopulation of platelets when stimulated with the combined agonists. The development of this platelet subpopulation is dependent on convulxin concentration and correlates with increases in factor Xa and thrombin generation. CONCLUSIONS: The development of increased coagulation factor binding to a subpopulation of platelets is not limited to alpha-granule components. Convulxin-induced increases in thrombin generation are regulated by the proportion of platelets with increased coagulation factor binding.

Full Text

Duke Authors

Cited Authors

  • Kempton, CL; Hoffman, M; Roberts, HR; Monroe, DM

Published Date

  • April 2005

Published In

Volume / Issue

  • 25 / 4

Start / End Page

  • 861 - 866

PubMed ID

  • 15653564

Pubmed Central ID

  • 15653564

Electronic International Standard Serial Number (EISSN)

  • 1524-4636

Digital Object Identifier (DOI)

  • 10.1161/01.ATV.0000155987.26583.9b

Language

  • eng

Conference Location

  • United States