Protein C inhibitor (plasminogen activator inhibitor-3) expression in the CWR22 prostate cancer xenograft.

Journal Article (Journal Article)

The serine protease inhibitor (serpin) protein C inhibitor (PCI) has been found in the prostate and possibly is a marker to distinguish normal prostate, benign prostatic hyperplasia, and prostate cancer. In this study, we assessed PCI expression in normal, hyperplastic, and malignant prostatic tissues, prostate cancer cell lines, and the CWR22 prostate cancer xenograft model that allowed us to study PCI expression and its regulation in response to androgens. By Northern blot, immunohistochemistry, and in situ hybridization, we found that PCI was expressed in both benign and malignant prostate tissues. Protein C inhibitor was expressed in both androgen-independent (PC-3) and androgen-dependent (LNCaP) prostate cancer cell lines. Furthermore, PCI was detected in all CWR22 tumor samples (androgen dependent, 6 days post-castration, 12 days post-castration followed by 72 h of testosterone treatment, and recurrent CWR22 tumor), although expression of the mature forms of both prostate-specific antigen (PSA) and its homolog, kallikrein 2 (hK2), was clearly androgen-dependent. These results suggest that PCI expression is not regulated by androgens and that PCI is unlikely to be a tumor suppressor gene, but also that PCI may be involved in regulating key serine proteases involved in metastatic prostate disease.

Full Text

Duke Authors

Cited Authors

  • Glasscock, LN; Réhault, SM; Gregory, CW; Cooper, ST; Jackson, TP; Hoffman, M; Church, FC

Published Date

  • August 2005

Published In

Volume / Issue

  • 79 / 1

Start / End Page

  • 23 - 32

PubMed ID

  • 15878512

International Standard Serial Number (ISSN)

  • 0014-4800

Digital Object Identifier (DOI)

  • 10.1016/j.yexmp.2005.02.008


  • eng

Conference Location

  • Netherlands