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Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells.

Publication ,  Journal Article
Asplin, IR; Misra, UK; Gawdi, G; Gonzalez-Gronow, M; Pizzo, SV
Published in: Arch Biochem Biophys
November 1, 2000

Cellular binding of receptor-recognized forms of alpha2-macroglobulin (alpha2M*) is mediated by the low-density lipoprotein receptor related protein (LRP) and the alpha2M signaling receptor (alpha2MSR). In nonmalignant cells, ligation of alpha2MSR promotes DNA synthesis and cellular proliferation. Here, we report that insulin treatment of highly metastatic 1-LN human prostate carcinoma selectively increases alpha2MSR expression and binding of alpha2M* to 1-LN cells. alpha2M* induces transient increases in intracellular calcium and inositol 1,4,5-trisphosphate in insulin-treated 1-LN cells, consistent with activation of alpha2MSR. Inhibition of signaling cascades activated by insulin blocks upregulation of alpha2MSR. By contrast, alpha2M* does not bind to nor induce intracellular signaling in PC-3 cells, even though 1-LN cells were subcloned from PC-3 cells. We suggest that alpha2M* behaves like a growth factor in these highly malignant cells. The 1-LN metastatic phenotype may result, in part, from aberrant expression of alpha2MSR, indicating the possible involvement of alpha2M* in tumor progression.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 1, 2000

Volume

383

Issue

1

Start / End Page

135 / 141

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Up-Regulation
  • Tumor Cells, Cultured
  • Signal Transduction
  • Receptors, Immunologic
  • RNA, Messenger
  • Prostatic Neoplasms
  • Neoplasm Metastasis
  • Neoplasm Invasiveness
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
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Asplin, I. R., Misra, U. K., Gawdi, G., Gonzalez-Gronow, M., & Pizzo, S. V. (2000). Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells. Arch Biochem Biophys, 383(1), 135–141. https://doi.org/10.1006/abbi.2000.2052
Asplin, I. R., U. K. Misra, G. Gawdi, M. Gonzalez-Gronow, and S. V. Pizzo. “Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells.Arch Biochem Biophys 383, no. 1 (November 1, 2000): 135–41. https://doi.org/10.1006/abbi.2000.2052.
Asplin IR, Misra UK, Gawdi G, Gonzalez-Gronow M, Pizzo SV. Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells. Arch Biochem Biophys. 2000 Nov 1;383(1):135–41.
Asplin, I. R., et al. “Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells.Arch Biochem Biophys, vol. 383, no. 1, Nov. 2000, pp. 135–41. Pubmed, doi:10.1006/abbi.2000.2052.
Asplin IR, Misra UK, Gawdi G, Gonzalez-Gronow M, Pizzo SV. Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells. Arch Biochem Biophys. 2000 Nov 1;383(1):135–141.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 1, 2000

Volume

383

Issue

1

Start / End Page

135 / 141

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Up-Regulation
  • Tumor Cells, Cultured
  • Signal Transduction
  • Receptors, Immunologic
  • RNA, Messenger
  • Prostatic Neoplasms
  • Neoplasm Metastasis
  • Neoplasm Invasiveness
  • Male