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Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.

Publication ,  Journal Article
Hoffman, M; Feldman, SR; Pizzo, SV
Published in: Biochim Biophys Acta
November 8, 1983

Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with human alpha 2-macroglobulin converted to its 'fast' form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Both alpha 2-macroglobulin-trypsin and alpha 2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2-) while native alpha 2-macroglobulin had little effect except at high concentration. The alpha 2-macroglobulin 'fast' forms, which bind with a Kd of about 8 nM, inhibited 50% generation of O2- (ID50) at a concentration of 7 nM while alpha 2-macroglobulin inhibited O2- production with an ID50 of 141 nM. The 'fast' forms of alpha 2-macroglobulin may play a role in the feedback regulation of inflammatory reactions.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

November 8, 1983

Volume

760

Issue

3

Start / End Page

421 / 423

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Superoxides
  • Mice
  • Macrophages
  • Macrophage Activation
  • In Vitro Techniques
  • Animals
  • 51 Physical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
 

Citation

APA
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ICMJE
MLA
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Hoffman, M., Feldman, S. R., & Pizzo, S. V. (1983). Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages. Biochim Biophys Acta, 760(3), 421–423. https://doi.org/10.1016/0304-4165(83)90384-7
Hoffman, M., S. R. Feldman, and S. V. Pizzo. “Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.Biochim Biophys Acta 760, no. 3 (November 8, 1983): 421–23. https://doi.org/10.1016/0304-4165(83)90384-7.
Hoffman M, Feldman SR, Pizzo SV. Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages. Biochim Biophys Acta. 1983 Nov 8;760(3):421–3.
Hoffman, M., et al. “Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.Biochim Biophys Acta, vol. 760, no. 3, Nov. 1983, pp. 421–23. Pubmed, doi:10.1016/0304-4165(83)90384-7.
Hoffman M, Feldman SR, Pizzo SV. Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages. Biochim Biophys Acta. 1983 Nov 8;760(3):421–423.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

November 8, 1983

Volume

760

Issue

3

Start / End Page

421 / 423

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Superoxides
  • Mice
  • Macrophages
  • Macrophage Activation
  • In Vitro Techniques
  • Animals
  • 51 Physical sciences
  • 31 Biological sciences
  • 06 Biological Sciences