Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.

Journal Article (Journal Article)

Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with human alpha 2-macroglobulin converted to its 'fast' form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Both alpha 2-macroglobulin-trypsin and alpha 2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2-) while native alpha 2-macroglobulin had little effect except at high concentration. The alpha 2-macroglobulin 'fast' forms, which bind with a Kd of about 8 nM, inhibited 50% generation of O2- (ID50) at a concentration of 7 nM while alpha 2-macroglobulin inhibited O2- production with an ID50 of 141 nM. The 'fast' forms of alpha 2-macroglobulin may play a role in the feedback regulation of inflammatory reactions.

Full Text

Duke Authors

Cited Authors

  • Hoffman, M; Feldman, SR; Pizzo, SV

Published Date

  • November 8, 1983

Published In

Volume / Issue

  • 760 / 3

Start / End Page

  • 421 - 423

PubMed ID

  • 6194826

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0304-4165(83)90384-7


  • eng

Conference Location

  • Netherlands