Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.
Journal Article (Journal Article)
Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with human alpha 2-macroglobulin converted to its 'fast' form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Both alpha 2-macroglobulin-trypsin and alpha 2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2-) while native alpha 2-macroglobulin had little effect except at high concentration. The alpha 2-macroglobulin 'fast' forms, which bind with a Kd of about 8 nM, inhibited 50% generation of O2- (ID50) at a concentration of 7 nM while alpha 2-macroglobulin inhibited O2- production with an ID50 of 141 nM. The 'fast' forms of alpha 2-macroglobulin may play a role in the feedback regulation of inflammatory reactions.
- Hoffman, M; Feldman, SR; Pizzo, SV
- November 8, 1983
Volume / Issue
- 760 / 3
Start / End Page
- 421 - 423
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)