Binding of alpha 2-macroglobulin to hepatocytes: mechanism of in vivo clearance.
Journal Article (Journal Article)
The binding of 125I-labeled human alpha 2-macroglobulin-methylamine to adult rat hepatocytes in primary culture was studied at 4 degrees C. Cells which had been in culture for 4 hours exhibited steady state ligand binding after 1 hour, a receptor number of 22,400 receptors per cell, and a dissociation constant of 0.6 nM. Adult rat hepatocytes exhibited a significant decrease in receptor number with increased time in primary culture with less than 10% of the initial number of receptors remaining after 2 days (p less than 0.01). In autopsy studies of mice injected intravenously with 125I-labeled alpha 2-macroglobulin-methylamine, greater than 90% of the cleared ligand was found in the liver. Autoradiography of the liver demonstrated that 80% of the ligand was cleared by hepatocytes. It is concluded that the hepatocytes are the primary pathway for clearance from the circulation of receptor recognized alpha 2-macroglobulin.
Full Text
Duke Authors
Cited Authors
- Feldman, SR; Rosenberg, MR; Ney, KA; Michalopoulos, G; Pizzo, SV
Published Date
- April 30, 1985
Published In
Volume / Issue
- 128 / 2
Start / End Page
- 795 - 802
PubMed ID
- 2581569
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1016/0006-291x(85)90117-2
Language
- eng
Conference Location
- United States