A colorimetric assay for releasable plasminogen activator.

Published

Journal Article

We describe an equilibrium assay for measuring release of plasminogen activator form blood-vessel walls and report data from 125 individuals free of overt thromboembolic disease. Excess human plasminogen is added to the euglobulin fraction of plasma obtained before and after venous occlusion at mean systolic pressure. To measure plasmin generation in these samples, we used the chromogenic plasmin substrate D-Val-Leu-Lys-p-nitroanilide, which liberates p-nitroaniline upon cleavage. Releasable plasminogen activator in 24 subjects was determined by this colorimetric assay and by the radiocasein assay previously reported by this laboratory (Am. J. Clin. Pathol. 76,403-409, 1981), and the results were compared. The correlation coefficient was 0.97. The colorimetric assay offers several advantages over the radiocasein assay: shorter incubation (6 vs 16 h) and no preparation or quantification of a radioactive substrate and its cleavage products.

Full Text

Duke Authors

Cited Authors

  • Campbell, EE; Shifman, MA; Lewis, JG; Pasqua, JJ; Pizzo, SV

Published Date

  • May 1, 1982

Published In

Volume / Issue

  • 28 / 5

Start / End Page

  • 1125 - 1128

PubMed ID

  • 6210467

Pubmed Central ID

  • 6210467

International Standard Serial Number (ISSN)

  • 0009-9147

Language

  • eng

Conference Location

  • England