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Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium.

Publication ,  Journal Article
Young, TN; Rodriguez, GC; Moser, TL; Bast, RC; Pizzo, SV; Stack, MS
Published in: Am J Obstet Gynecol
May 1994

OBJECTIVE: Because elevated expression and cell surface association of urinary-type plasminogen activator have been linked to invasive potential in certain tumor types, we examined the expression of urinary-type plasminogen activator and urinary-type plasminogen activator receptor in ovarian epithelial carcinoma tissues and cells as compared with normal ovarian epithelium. STUDY DESIGN: Monoclonal antibodies specific for urinary-type plasminogen activator and urinary-type plasminogen activator receptor were used for immunohistochemical staining of tissues and cells to assess expression of these antigens in frozen sections of normal and tumor tissue. Substrate zymography was used to detect plasminogen activator activity in ovarian carcinoma ascites and in conditioned media of cultured cells, whereas a Western blot assay was used to identify urinary-type plasminogen activator receptor in cultured cells. RESULTS: Normal ovarian epithelium expressed urinary-type plasminogen activator receptor (4/4 positive) but little or no urinary-type plasminogen activator (0/4 positive), whereas epithelial ovarian carcinomas frequently expressed urinary-type plasminogen activator (4/8 positive) in conjunction with urinary-type plasminogen activator receptor (7/9 positive). High levels of urinary-type plasminogen activator were detected in 15 of 19 samples of ascites. DOV 13, OVCA 420, OVCA 429, OVCA 432, and OVCA 433 cell lines secreted urinary-type plasminogen activator in variable quantities, whereas normal ovarian epithelial cells did not secrete any detectable plasminogen activator. Urinary-type plasminogen activator receptor had similar levels of expression in all cancer cell lines and normal ovarian epithelium. CONCLUSION: Overexpression of urinary-type plasminogen activator is associated with malignant transformation of the ovarian epithelium. Increased cell surface proteolysis mediated by urinary-type plasminogen activator bound to cell surface urinary-type plasminogen activator receptor may contribute to metastatic behavior in ovarian carcinoma.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

May 1994

Volume

170

Issue

5 Pt 1

Start / End Page

1285 / 1296

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Plasminogen Activators
  • Ovary
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Young, T. N., Rodriguez, G. C., Moser, T. L., Bast, R. C., Pizzo, S. V., & Stack, M. S. (1994). Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium. Am J Obstet Gynecol, 170(5 Pt 1), 1285–1296. https://doi.org/10.1016/s0002-9378(94)70143-1
Young, T. N., G. C. Rodriguez, T. L. Moser, R. C. Bast, S. V. Pizzo, and M. S. Stack. “Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium.Am J Obstet Gynecol 170, no. 5 Pt 1 (May 1994): 1285–96. https://doi.org/10.1016/s0002-9378(94)70143-1.
Young TN, Rodriguez GC, Moser TL, Bast RC, Pizzo SV, Stack MS. Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium. Am J Obstet Gynecol. 1994 May;170(5 Pt 1):1285–96.
Young, T. N., et al. “Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium.Am J Obstet Gynecol, vol. 170, no. 5 Pt 1, May 1994, pp. 1285–96. Pubmed, doi:10.1016/s0002-9378(94)70143-1.
Young TN, Rodriguez GC, Moser TL, Bast RC, Pizzo SV, Stack MS. Coordinate expression of urinary-type plasminogen activator and its receptor accompanies malignant transformation of the ovarian surface epithelium. Am J Obstet Gynecol. 1994 May;170(5 Pt 1):1285–1296.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

May 1994

Volume

170

Issue

5 Pt 1

Start / End Page

1285 / 1296

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Plasminogen Activators
  • Ovary
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female