An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus.

Published

Journal Article

The importance of E2A transcription factors in T cell development has been demonstrated in studies of E2A-deficient mice, which display abnormal T cell development and a high frequency of T cell lymphomas. Because E2A expression is not restricted to the T cell lineage, the primary cause of the T cell phenotype in E2A-deficient mice was not fully determined. To further investigate the role of E2A in T cell lineage, we generated mice with the E2A gene disrupted exclusively during thymocyte development using the Cre-lox system. We show that this system allows E2A gene disruption to occur throughout the double-negative stage of thymocyte development. E2A deletion appears to be completed before development reaches the double-positive stage. Consistent with the gene disruption, these mice reveal a T cell intrinsic role for E2A during the transition from the double-negative stage to the double-positive stage of thymocyte development. In contrast to germline E2A knockout mice, conditional E2A knockout mice do not develop T cell lymphoma. This work establishes a new model for further investigating E2A function in T cell development and leukemiogenesis.

Full Text

Duke Authors

Cited Authors

  • Pan, L; Hanrahan, J; Li, J; Hale, LP; Zhuang, Y

Published Date

  • April 15, 2002

Published In

Volume / Issue

  • 168 / 8

Start / End Page

  • 3923 - 3932

PubMed ID

  • 11937548

Pubmed Central ID

  • 11937548

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.168.8.3923

Language

  • eng

Conference Location

  • United States