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Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor.

Publication ,  Journal Article
Kraus, WE; Longabaugh, JP; Liggett, SB
Published in: Am J Physiol
August 1992

Continuous electrical pacing (EP) at 10 Hz of the peroneal nerve innervating fast-twitch muscles of the hindlimb in adult rabbits increases skeletal muscle concentrations of adenosine 3',5'-cyclic monophosphate (cAMP) by 3.1-fold at 10 days and increases beta-adrenergic receptor (beta-AR) density by 2.0-fold at 21 days. To determine whether beta-AR, the alpha-subunit of guanine nucleotide proteins (Gs alpha), or adenylyl cyclase is primarily responsible for pacing-induced increases in muscle cAMP, we measured adenylyl cyclase activity (ACA) in muscles that were electrically paced for 3 (n = 4), 10 (n = 8), and 21 (n = 8) days. EP resulted in a time-dependent increase in ACA that was 2.2 +/- 0.3-fold (P less than 0.005) at 21 days. EP significantly increased GTP-, 5'-guanylylimidodiphosphate-, isoproterenol-, NaF-, and forskolin-stimulated ACA, and propranolol administration to rabbits during EP did not alter pacing-induced changes in ACA. There were no changes in protein concentration, Na(+)-K(+)-adenosinetriphosphatase activity, or Gs alpha with EP. Based on these studies, we conclude that EP appears to increase cAMP through mechanisms independent of the beta-AR and through mechanisms that may involve alterations at the level of adenylyl cyclase.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

August 1992

Volume

263

Issue

2 Pt 1

Start / End Page

E226 / E230

Location

United States

Related Subject Headings

  • Time Factors
  • Sodium-Potassium-Exchanging ATPase
  • Receptors, Adrenergic, beta
  • Rabbits
  • Muscles
  • Membrane Proteins
  • GTP-Binding Proteins
  • Electric Stimulation
  • Cardiovascular System & Hematology
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Kraus, W. E., Longabaugh, J. P., & Liggett, S. B. (1992). Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor. Am J Physiol, 263(2 Pt 1), E226–E230. https://doi.org/10.1152/ajpendo.1992.263.2.E226
Kraus, W. E., J. P. Longabaugh, and S. B. Liggett. “Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor.Am J Physiol 263, no. 2 Pt 1 (August 1992): E226–30. https://doi.org/10.1152/ajpendo.1992.263.2.E226.
Kraus WE, Longabaugh JP, Liggett SB. Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor. Am J Physiol. 1992 Aug;263(2 Pt 1):E226–30.
Kraus, W. E., et al. “Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor.Am J Physiol, vol. 263, no. 2 Pt 1, Aug. 1992, pp. E226–30. Pubmed, doi:10.1152/ajpendo.1992.263.2.E226.
Kraus WE, Longabaugh JP, Liggett SB. Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the beta-adrenergic receptor. Am J Physiol. 1992 Aug;263(2 Pt 1):E226–E230.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

August 1992

Volume

263

Issue

2 Pt 1

Start / End Page

E226 / E230

Location

United States

Related Subject Headings

  • Time Factors
  • Sodium-Potassium-Exchanging ATPase
  • Receptors, Adrenergic, beta
  • Rabbits
  • Muscles
  • Membrane Proteins
  • GTP-Binding Proteins
  • Electric Stimulation
  • Cardiovascular System & Hematology
  • Animals