Skip to main content
Journal cover image

Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ.

Publication ,  Journal Article
Atkins, BZ; Lewis, CW; Kraus, WE; Hutcheson, KA; Glower, DD; Taylor, DA
Published in: Ann Thorac Surg
January 1999

BACKGROUND: Adult heart lacks stem cells and cannot effectively regenerate. In contrast, skeletal muscle is constantly undergoing repair. We proposed to transplant immature skeletal myoblasts into injured myocardium. METHODS: Approximately 7x10(6) soleus skeletal myoblasts were expanded in vitro from adult New Zealand White rabbits (n = 23) whose posterior left ventricle was cryoinjured to create a transmural lesion. Autologous myoblasts (n = 18) or saline (n = 5) was transplanted into the central cryolesion at the time of injury (n = 6) or 1 week later (n = 12). Hearts were harvested 2 weeks after injection. RESULTS: Myoblast transfer did not incur further morbidity. After cryolesion, grossly, a 1.6-cm epicardial hemorrhagic lesion could be seen. Histologically, the transmural lesion contained inflammatory cells and active scarring but no viable cardiomyocytes. Electron microscopy demonstrated a predominance of collagen and fibroblasts. Nine hearts contained multinucleated cells within the cryolesion that covered approximately 75% of the central cryolesion in 17% of animals. Immunohistochemical analysis confirmed their skeletal muscle origin. At the periphery of the lesion, isolated clusters of nonskeletal muscle cells could be visualized (n = 12) that resembled immature cardiocytes. CONCLUSIONS: Autologous skeletal myoblasts can regenerate viable striated tissue within damaged myocardium. Myoblast transfer warrants further investigation as a new method for improving myocardial performance within infarcted myocardium.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Ann Thorac Surg

DOI

ISSN

0003-4975

Publication Date

January 1999

Volume

67

Issue

1

Start / End Page

124 / 129

Location

Netherlands

Related Subject Headings

  • Transplantation, Autologous
  • Respiratory System
  • Regeneration
  • Rabbits
  • Myocardium
  • Muscle, Skeletal
  • Immunohistochemistry
  • Cell Transplantation
  • Cardiomyoplasty
  • Biopsy
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Atkins, B. Z., Lewis, C. W., Kraus, W. E., Hutcheson, K. A., Glower, D. D., & Taylor, D. A. (1999). Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ. Ann Thorac Surg, 67(1), 124–129. https://doi.org/10.1016/s0003-4975(98)01197-7
Atkins, B. Z., C. W. Lewis, W. E. Kraus, K. A. Hutcheson, D. D. Glower, and D. A. Taylor. “Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ.Ann Thorac Surg 67, no. 1 (January 1999): 124–29. https://doi.org/10.1016/s0003-4975(98)01197-7.
Atkins BZ, Lewis CW, Kraus WE, Hutcheson KA, Glower DD, Taylor DA. Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ. Ann Thorac Surg. 1999 Jan;67(1):124–9.
Atkins, B. Z., et al. “Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ.Ann Thorac Surg, vol. 67, no. 1, Jan. 1999, pp. 124–29. Pubmed, doi:10.1016/s0003-4975(98)01197-7.
Atkins BZ, Lewis CW, Kraus WE, Hutcheson KA, Glower DD, Taylor DA. Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ. Ann Thorac Surg. 1999 Jan;67(1):124–129.
Journal cover image

Published In

Ann Thorac Surg

DOI

ISSN

0003-4975

Publication Date

January 1999

Volume

67

Issue

1

Start / End Page

124 / 129

Location

Netherlands

Related Subject Headings

  • Transplantation, Autologous
  • Respiratory System
  • Regeneration
  • Rabbits
  • Myocardium
  • Muscle, Skeletal
  • Immunohistochemistry
  • Cell Transplantation
  • Cardiomyoplasty
  • Biopsy