Specific photoaffinity labelling of inhibitory adenosine receptors.
Published
Journal Article
N6(L-phenylisopropyl)adenosine (L-PIA) and N6(3-iodo-4-azido benzyl)-adenosine (IAzBA) inhibit the adenylate cyclase activity in synaptic membranes of chick cerebellum via Ri adenosine receptors. [3H]L-PIA and [125I]AzBA bind to these membranes with Kd values of approximately 1 nM and Bmax values of approximately 1000 fmol/mg protein. Photolysis of [125I]AzBA bound to synaptic membranes results in the specific incorporation of radioactivity into a protein with Mr = 36,000. This photoincorporation is blocked by simultaneous exposure to L-PIA, theophylline, an adenosine receptor antagonist, or Gpp(NH)p, but not by cytosine, suggesting that the 36,000 dalton protein is the Ri adenosine receptor or a subunit of the receptor that contains the adenosine binding site.
Full Text
Duke Authors
Cited Authors
- Choca, JI; Kwatra, MM; Hosey, MM; Green, RD
Published Date
- August 30, 1985
Published In
Volume / Issue
- 131 / 1
Start / End Page
- 115 - 121
PubMed ID
- 2994642
Pubmed Central ID
- 2994642
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1016/0006-291x(85)91778-4
Language
- eng
Conference Location
- United States