Specific photoaffinity labelling of inhibitory adenosine receptors.

Journal Article (Journal Article)

N6(L-phenylisopropyl)adenosine (L-PIA) and N6(3-iodo-4-azido benzyl)-adenosine (IAzBA) inhibit the adenylate cyclase activity in synaptic membranes of chick cerebellum via Ri adenosine receptors. [3H]L-PIA and [125I]AzBA bind to these membranes with Kd values of approximately 1 nM and Bmax values of approximately 1000 fmol/mg protein. Photolysis of [125I]AzBA bound to synaptic membranes results in the specific incorporation of radioactivity into a protein with Mr = 36,000. This photoincorporation is blocked by simultaneous exposure to L-PIA, theophylline, an adenosine receptor antagonist, or Gpp(NH)p, but not by cytosine, suggesting that the 36,000 dalton protein is the Ri adenosine receptor or a subunit of the receptor that contains the adenosine binding site.

Full Text

Duke Authors

Cited Authors

  • Choca, JI; Kwatra, MM; Hosey, MM; Green, RD

Published Date

  • August 30, 1985

Published In

Volume / Issue

  • 131 / 1

Start / End Page

  • 115 - 121

PubMed ID

  • 2994642

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(85)91778-4


  • eng

Conference Location

  • United States