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Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor.

Publication ,  Journal Article
Lee, K; Richardson, CD; Razik, MA; Kwatra, MM; Schwinn, DA
Published in: DNA Seq
March 1998

In spite of their critical importance in myocardial hypertrophy and benign prostatic hyperplasia, nothing is known about mechanisms underlying transcriptional regulation of alpha 1a-adrenergic receptors (alpha 1aARs). Therefore we cloned 6.2 kb of novel sequence upstream of the initiator ATG in the human alpha 1aAR gene. Sequence analysis reveals a TATA-less promoter, the presence of several initiator (Inr) consensus sequences, multiple GC rich regions consistent with Sp-1 binding, and consensus sequences for AP-1 and AP-2 as well as putative cis transcriptional regulatory elements for binding of CREB (cyclic-AMP response element binding protein), glucocorticoids, estrogen, and insulin. Compared to the alpha 1bAR, the alpha 1aAR has several more cis regulatory elements, suggesting more complex regulation. The importance of alpha 1aARs in human disease makes it imperative to determine mechanisms underlying transcription and ultimately expression of this receptor. These studies can now be undertaken with the availability of human alpha 1aAR 5'-flanking and 5'-untranslated sequence.

Duke Scholars

Published In

DNA Seq

DOI

ISSN

1042-5179

Publication Date

March 1998

Volume

8

Issue

4

Start / End Page

271 / 276

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Sequence Analysis, DNA
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Adrenergic, alpha-1
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Humans
  • Genetics & Heredity
  • Gene Expression Regulation
  • Base Sequence
 

Citation

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MLA
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Lee, K., Richardson, C. D., Razik, M. A., Kwatra, M. M., & Schwinn, D. A. (1998). Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor. DNA Seq, 8(4), 271–276. https://doi.org/10.3109/10425179809008464
Lee, K., C. D. Richardson, M. A. Razik, M. M. Kwatra, and D. A. Schwinn. “Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor.DNA Seq 8, no. 4 (March 1998): 271–76. https://doi.org/10.3109/10425179809008464.
Lee K, Richardson CD, Razik MA, Kwatra MM, Schwinn DA. Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor. DNA Seq. 1998 Mar;8(4):271–6.
Lee, K., et al. “Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor.DNA Seq, vol. 8, no. 4, Mar. 1998, pp. 271–76. Pubmed, doi:10.3109/10425179809008464.
Lee K, Richardson CD, Razik MA, Kwatra MM, Schwinn DA. Multiple potential regulatory elements in the 5' flanking region of the human alpha 1a-adrenergic receptor. DNA Seq. 1998 Mar;8(4):271–276.
Journal cover image

Published In

DNA Seq

DOI

ISSN

1042-5179

Publication Date

March 1998

Volume

8

Issue

4

Start / End Page

271 / 276

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Sequence Analysis, DNA
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Adrenergic, alpha-1
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Humans
  • Genetics & Heredity
  • Gene Expression Regulation
  • Base Sequence